Pinheiro Breno Queiroz, de Magalhães Francisco Felipe, Alves Francisco Wesley da Silva, Silva Isaac Neto Goes, Faustino Augusto Manuel Rodrigues, da Silva Lúcia Daniel Machado
Laboratório de Reprodução de Carnívoros, Faculdade de Veterinária, Universidade Estadual do Ceará, Fortaleza, CE, Brasil.
Laboratório de Patologia Clínica Veterinária, Faculdade de Veterinária, Universidade Estadual do Ceará, Fortaleza, CE, Brasil.
Anim Reprod. 2023 Mar 13;20(1):e20220086. doi: 10.1590/1984-3143-AR2022-0086. eCollection 2023.
The identification of putative prognostic factors in canine mammary neoplasms (CMNs) has been focused on tissue-specific biomarkers, but the serum biomarkers, including cancer antigen 15-3 (CA 15-3), c-reactive protein (CRP), and lactate dehydrogenase (LDH) have been demonstrated to display clinical application in cases of CMNs. The aim of the study was to evaluate the levels of these serum biomarkers and their association with well-established prognostic factors in CMNs. Samples from 15 female canines with CMNs and 15 clinically healthy ones were collected. The results were evaluated using the Tukey's, Pearson, or Spearman tests. The cut-off point, sensitivity, specificity, and area under curve (AUC) were evaluated using the receiver operating characteristic (ROC) curve analysis in a logistic regression model (P<0.05). The levels of CA 15-3, CRP and LDH were significantly higher in the serum of female dogs with CMNs compared to the healthy ones. Moreover, these factors were positively correlated with ulceration, tumor size, histopathological grade, metastatic lymph node, and clinical staging. Female dogs with CMNs were found to exhibit highest serum levels of CA 15-3, CRP, and LDH. Therefore, they can be applied to improve the efficacy of the diagnosis and prognostic evaluation in casas of CMNs.
犬乳腺肿瘤（CMNs）中假定预后因素的鉴定一直聚焦于组织特异性生物标志物，但血清生物标志物，包括癌抗原15-3（CA 15-3）、C反应蛋白（CRP）和乳酸脱氢酶（LDH），已被证明在CMNs病例中具有临床应用价值。本研究的目的是评估这些血清生物标志物的水平及其与CMNs中已确立的预后因素的关联。收集了15只患有CMNs的雌性犬和15只临床健康犬的样本。使用Tukey检验、Pearson检验或Spearman检验对结果进行评估。在逻辑回归模型中，使用受试者工作特征（ROC）曲线分析评估截断点、敏感性、特异性和曲线下面积（AUC）（P<0.05）。与健康犬相比，患有CMNs的雌性犬血清中CA 15-3、CRP和LDH的水平显著更高。此外，这些因素与溃疡、肿瘤大小、组织病理学分级、转移淋巴结和临床分期呈正相关。发现患有CMNs的雌性犬血清中CA 15-3、CRP和LDH的水平最高。因此，它们可用于提高CMNs病例的诊断和预后评估效果。
