低CD4/CD8比值所反映的临床症状和免疫损伤应增加对HIV合并结核病感染的怀疑。
Clinical symptoms and immune injury reflected by low CD4/CD8 ratio should increase the suspicion of HIV coinfection with tuberculosis.
作者信息
Li Li, Abudureheman Zulipikaer, Zhong XueMei, Gong Hui, Yang Fan, Awuti Abuduweili, Alimu Ayiguli, Yilamujiang Subinuer, Zheng DaYong, Zou XiaoGuang
机构信息
Department of Respiratory and Critical Care Medicine, First People's Hospital of Kashi, Kashi, China.
State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Xinjiang Medical University, Urumqi, China.
出版信息
Heliyon. 2023 Mar 4;9(3):e14219. doi: 10.1016/j.heliyon.2023.e14219. eCollection 2023 Mar.
BACKGROUND
Patients who are coinfected with human immunodeficiency virus 1 (HIV) and (TB) benefit from timely diagnosis and treatment. In the present study frequencies of CD3, CD4, and CD8 T cells among peripheral blood mononuclear cells (PBMCs) of patients in the Kashi region of China infected with HIV, TB, and both HIV and TB (HIV-TB) were investigated to provide a basis for rapid identification of coinfected patients.
METHODS
A total of 62 patients with HIV, TB, or HIV-TB who were first hospitalized at our institution were included in the study, as were 30 controls. PBMCs were isolated, and the frequencies of CD3, CD4, and CD8 T cells were determined via flow cytometry.
RESULTS
The frequency of CD4 T cells and the CD4/CD8 ratio were significantly lower in the HIV-TB group than in the other three groups. In fever patients the frequency of CD4 T cells and the CD4/CD8 ratio were significantly lower in the HIV-TB group than in the HIV group and the TB group. In patients who exhibited rapid weight loss there were no significant differences in the frequency of CD4 T cells or the CD4/CD8 ratio between the groups. The results of treatment were compared in the HIV, TB, and HIV-TB groups after 7 days, and there were obvious improvements in the frequency of CD4 T cells and the CD4/CD8 ratio.
CONCLUSION
Clinical symptoms and the degree of immune injury can heighten suspicion for HIV-TB coinfection.
背景
合并感染人类免疫缺陷病毒1型（HIV）和结核病（TB）的患者可从及时诊断和治疗中获益。在本研究中，对中国喀什地区感染HIV、TB以及同时感染HIV和TB（HIV-TB）患者外周血单个核细胞（PBMC）中CD3、CD4和CD8 T细胞的频率进行了调查，以便为快速识别合并感染患者提供依据。
方法
本研究纳入了62例首次在我院住院的HIV、TB或HIV-TB患者以及30名对照。分离PBMC，并通过流式细胞术测定CD3、CD4和CD8 T细胞的频率。
结果
HIV-TB组的CD4 T细胞频率和CD4/CD8比值显著低于其他三组。发热患者中，HIV-TB组的CD4 T细胞频率和CD4/CD8比值显著低于HIV组和TB组。体重快速下降的患者中，各组间CD4 T细胞频率或CD4/CD8比值无显著差异。7天后比较HIV组、TB组和HIV-TB组的治疗结果，CD4 T细胞频率和CD4/CD8比值有明显改善。
结论
临床症状和免疫损伤程度可增加对HIV-TB合并感染的怀疑。
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