抗兔补体第五成分（C5）单克隆抗体的制备与鉴定

Preparation and characterization of monoclonal antibodies against the fifth component of rabbit complement (C5).

作者信息

Giclas P C, Baker S L, Gillespie M L, Wilcox C

机构信息

Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206.

出版信息

J Immunol Methods. 1987 Dec 24;105(2):201-9. doi: 10.1016/0022-1759(87)90267-5.

DOI:10.1016/0022-1759(87)90267-5

PMID:3693908

Abstract

By immunizing mice genetically deficient in C5 we were able to obtain a group of monoclonal antibodies to rabbit C5 that cross-react with C5 from a wide variety of mammalian sera, including mouse. The specificity of the monoclonal antibodies was against native C5 and C5b but not C5a. The antibodies strongly inhibit the expression of C5 hemolytic activity. We suggest that these monoclonal antibodies will be useful for studying C5 as well as providing a way to selectively deplete C5 from plasma in vitro or in vivo.

摘要

通过对C5基因缺陷的小鼠进行免疫，我们得以获得一组针对兔C5的单克隆抗体，这些抗体可与多种哺乳动物血清（包括小鼠血清）中的C5发生交叉反应。这些单克隆抗体的特异性针对天然C5和C5b，而非C5a。这些抗体强烈抑制C5溶血活性的表达。我们认为，这些单克隆抗体将有助于研究C5，并提供一种在体外或体内从血浆中选择性去除C5的方法。