Program in Cellular, Molecular, and Microbial Biology, Division of Biological Sciences, University of Montana, Missoula, Montana, USA.
Entomology Branch, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
Vector Borne Zoonotic Dis. 2023 Jun;23(6):324-330. doi: 10.1089/vbz.2022.0087. Epub 2023 Mar 20.
is a recently described species endemic to Peru, where it causes verruga peruana in humans. While the arthropod vector of transmission is unknown, human coinfections with suggest that phlebotomine sand flies are a vector. To address the hypothesis that sand flies are involved in the bacterium's transmission, sand flies were used as an infection model, together with green fluorescent protein-expressing . Results showed that bacterial infections were clearly established, limited to the anterior midgut of the female fly, and maintained for roughly 7 days. At 3-7 days postinfection, a prominent microcolony of aggregated bacteria was observed in the anterior midgut, immediately distal to the stomodeal valve of the esophagus. In contrast, eggs, diuretic fluid, feces, and other tissues were not infected. These results suggest that certain sand fly species within the endemic zone for may play a role in the bacterium's maintenance and possibly in its transmission to humans.
是一种最近在秘鲁发现的物种，在秘鲁引起秘鲁疣。虽然 的传播的节肢动物媒介尚不清楚，但人类与 的合并感染表明，白蛉沙蝇可能是一种媒介。为了验证沙蝇是否参与了细菌的传播，使用沙蝇作为感染模型，同时使用表达绿色荧光蛋白的 。结果表明，细菌感染明显建立，仅限于雌性蝇的前中肠，并维持大约 7 天。在感染后 3-7 天，在前中肠中观察到聚集细菌的明显微菌落，位于食管的口前阀的远端。相比之下，卵、利尿剂、粪便和其他组织没有被感染。这些结果表明，在 的流行区域内的某些沙蝇物种可能在细菌的维持中发挥作用，并且可能在其向人类传播中发挥作用。
