Todorović Jasna, Dinčić Marko, Krstić Danijela Z, Čolović Mirjana B, Ostojić Jelena Nešović, Kovačević Sanjin, Lopičić Srđan, Spasić Svetolik, Brkić Predrag, Milovanović Aleksandar
University of Belgrade, Faculty of Medicine, Institute of Pathological Physiology, Serbia.
University of Belgrade, Faculty of Medicine, Institute of Medical Chemistry, Serbia.
Sleep Med. 2023 May;105:14-20. doi: 10.1016/j.sleep.2023.03.002. Epub 2023 Mar 6.
Thyroid dysfunctions as well as sleep abnormalities are usually followed by neurological, psychiatric and/or behavioral disorders. On the other hand, changes in the brain adenosine triphosphatases (ATPases) and acetylcholinesterase (AChE) activities show significant importance in pathogenetic pathways in the evolution of numerous neuropsychiatric diseases.
This study aimed to evaluate the in vivo simultaneous effects of hypothyroidism and paradoxical sleep deprivation for 72 h on synaptosomalATPases and AChE activities of whole rat brains. In order to induce hypothyroidism, 6-n-propyl-2-thiouracil was administrated in drinking water during 21 days. The modified multiple platform method was used to induce paradoxical sleep deprivation. The AChE and ATPases activities were measured using spectrophotometric methods.
Hypothyroidism significantly increased the activity of Na/K-ATPase compared to other groups, while at the same time significantly decreased AChE activity compared to the CT and SD groups. Paradoxical sleep deprivation significantly increased AChE activity compared to other groups. The simultaneous effect of hypothyroidism and sleep deprivation reduced the activity of all three enzymes (for Na/K-ATPase between HT/SD and HT group p < 0.0001, SD group p < 0.001,CT group p = 0.013; for ecto-ATPases between HT/SD and HT group p = 0.0034, SD group p = 0.0001, CT group p = 0.0007; for AChE between HT/SD and HT group p < 0.05, SD group p < 0.0001, CT group p < 0.0001).
The effect of simultaneous existence of hypothyroidism and paradoxical sleep deprivation reduces the activity of the Na/K-ATPase, ecto-ATPases, and AChE, what is different from individual effect of hypothyroidism and paradoxical sleep deprivation itself. This knowledge could help in the choice of appropriate therapy in such condition.
