Clinic of Nuclear Medicine, Jena University Hospital, Am Klinikum 1, 07747 Jena, Germany.
Clinic of Nuclear Medicine, Jena University Hospital, Am Klinikum 1, 07747 Jena, Germany.
Nucl Med Biol. 2023 Mar-Apr;118-119:108333. doi: 10.1016/j.nucmedbio.2023.108333. Epub 2023 Mar 15.
With the introduction of automated synthetization methods, the in-house production of several Ga-based tracers became feasible in hospital laboratories. We describe a possible standard operating procedure (SOP) for [Ga]Ga-oxine-labeled heat-denaturated erythrocytes, which can be used for selective imaging in patients with splenic disorders.
Heat-denaturated erythrocytes were labeled with [Ga]Ga-oxine, which was produced from Ga and 8-hydroxyquinoline on an automated synthesizer. The workflow was validated in a good manufacturing/good radiopharmaceutical practice (GMP/GRP) certified laboratory. A patient underwent [Ga]Ga-oxine-erythrocyte PET/CT for differentiation of an intrapancreatic mass.
[Ga]Ga-oxine and [Ga]Ga-oxine-labeled erythrocytes could be synthesized reproducibly and reliably. The products met GMP quality standards. The tracer showed high accumulation in the intrapancreatic mass, consistent with an accessory spleen.
PET/CT imaging with [Ga]Ga-oxine-labeled, heat-denaturated erythrocytes can be a backup method for the differentiation of functioning splenic tissue from tumors. An SOP for the production of the tracer in a clinical setting could be established.
随着自动化合成方法的引入，医院实验室可以实现几种基于镓的示踪剂的内部生产。我们描述了一种用于[Ga]Ga-oxine 标记的热变性红细胞的可能标准操作程序（SOP），该程序可用于脾脏疾病患者的选择性成像。
热变性红细胞用[Ga]Ga-oxine 标记，该物质是在自动化合成器上由镓和 8-羟基喹啉合成的。该工作流程在经过良好生产/良好放射性药物实践（GMP/GRP）认证的实验室中进行了验证。一名患者接受了[Ga]Ga-oxine-红细胞 PET/CT 检查，以区分胰腺内肿块。
可以重复且可靠地合成[Ga]Ga-oxine 和[Ga]Ga-oxine 标记的红细胞。产品符合 GMP 质量标准。示踪剂在胰腺内肿块中高度积聚，与副脾一致。
用[Ga]Ga-oxine 标记的热变性红细胞进行 PET/CT 成像可以作为区分功能性脾组织与肿瘤的后备方法。可以在临床环境中建立生产示踪剂的 SOP。
