Institute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035 Kórnik, Poland.
Department of Chemistry, Faculty of Agriculture and Forestry, University of Warmia and Mazury in Olsztyn, Plac Łódzki 4, 10-719 Olsztyn, Poland.
Tree Physiol. 2023 Jul 9;43(7):1142-1158. doi: 10.1093/treephys/tpad036.
The problems posed by seed sensitivity to desiccation and aging have motivated the development of various techniques for mitigating their detrimental effects. The redox priming of seeds in antioxidant solution to improve their postharvest performance is one of the approaches. Spermidine (Spd) was tested as an invigorating solution on nondormant recalcitrant (desiccation-sensitive) seeds of the silver maple (Acer saccharinum L.). The treatment resulted in an 8-10% increase in germination capacity in seeds subjected to mild and severe desiccation, while in aged seeds stored for 6 months, no significant change was observed. The cellular redox milieu, genetic stability, mitochondrial structure and function were investigated to provide information about the cellular targets of Spd activity. Spermidine improved the antioxidative capacity, especially the activity of catalase, and cellular membrane stability, protected genome integrity from oxidative damage and increased the efficiency of mitochondria. However, it also elicited a hydrogen peroxide burst. Therefore, it seems that redox priming in nondormant seeds that are highly sensitive to desiccation, although it positively affected desiccated seed performance, may not be a simple solution to reinvigorate stored seeds with a low-efficiency antioxidant system.
种子对干燥和衰老的敏感性所带来的问题，促使人们开发出各种技术来减轻这些不利影响。用抗氧化溶液对种子进行氧化还原引发处理，以提高其采后性能就是其中一种方法。本文将亚精胺（Spd）作为一种激活剂，测试其对非休眠性顽拗（干燥敏感）种子（银枫，Acer saccharinum L.）的效果。结果表明，轻度和重度干燥处理后的种子发芽能力提高了 8-10%，而经过 6 个月贮藏的老化种子则没有明显变化。研究了细胞氧化还原环境、遗传稳定性、线粒体结构和功能，以提供有关 Spd 活性的细胞靶标的信息。亚精胺提高了抗氧化能力，特别是过氧化氢酶的活性和细胞膜的稳定性，保护基因组免受氧化损伤，并提高了线粒体的效率。然而，它也引发了过氧化氢的爆发。因此，对于对干燥高度敏感的非休眠种子来说，氧化还原引发处理虽然可以改善干燥种子的性能，但对于具有低效抗氧化系统的贮藏种子来说，可能并不是一个简单的复壮方法。
