Department of Biology, Kavian Institute of Higher Education, Mashhad, Iran.
Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Bioprocess Biosyst Eng. 2023 May;46(5):727-734. doi: 10.1007/s00449-023-02859-4. Epub 2023 Mar 22.
In this study, Gum Arabic natural polymer was used to green synthesize calcium carbonate nanoparticles (CaCO-NPs). Synthesized CaCO-NPs were identified using various analyses such as FTIR, XRD, FESEM, EDX, and UV-Vis. The UV spectrum of nanoparticles showed an absorption band at a wavelength of 320 nm. FTIR analysis also confirmed the synthesis of nanoparticles. XRD studies showed that CaCO-NPs have a rhombohedral crystalline structure with space group R-3c and an average size of about 42 nm. FESEM images showed that CaCO-NPs have cubic morphology and EDX results confirmed the presence of carbon, calcium, and oxygen elements. The synthesized CaCO-NPs showed good photocatalytic activity to methylene blue (MB) dye degradation, which percentage degradation was 93% after 120 min. Also, the cytotoxicity of CaCO-NPs has been examined on the normal L929 and cancer CT26 cell lines and the IC value was about 250 µg/mL for cancer cells.
在这项研究中，我们使用天然树胶阿拉伯胶来绿色合成碳酸钙纳米粒子（CaCO-NPs）。通过傅里叶变换红外光谱（FTIR）、X 射线衍射（XRD）、场发射扫描电子显微镜（FESEM）、能谱分析（EDX）和紫外-可见分光光度计（UV-Vis）等多种分析方法对合成的 CaCO-NPs 进行了鉴定。纳米粒子的紫外光谱在 320nm 波长处显示出吸收带。FTIR 分析也证实了纳米粒子的合成。XRD 研究表明，CaCO-NPs 具有菱面体晶型结构，空间群为 R-3c，平均粒径约为 42nm。FESEM 图像显示 CaCO-NPs 具有立方形态，EDX 结果证实了碳、钙和氧元素的存在。合成的 CaCO-NPs 对亚甲基蓝（MB）染料的光催化降解表现出良好的活性，在 120 分钟后，降解率达到 93%。此外，我们还研究了 CaCO-NPs 对正常 L929 和癌细胞 CT26 细胞系的细胞毒性，其对癌细胞的 IC 值约为 250μg/mL。
