Huang Yanping, Song Xianbing, Yang Jun, Wang Taorong, Zhang Ye, Chen Xiaoyu, Ye Jing
Department of Human Anatomy, Histology & Embryology, Anhui Medical College, Hefei 230601, China.
Department of of Pathology, Anhui Medical College, Hefei 230601, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2023 Mar;39(3):236-241.
Objective To investigate the anti-inflammatory effects of sinoline on adjuvant arthritis (AA) rats and the changes of NOD like receptor pyrin-domain containing 3 (NLRP3). Methods Wistar rats were randomly divided into control group, AA model group, (100, 200, 400) mg/kg sinomenine group and 100 mg/kg Tripterygium wilfordii group, with 10 rats in each group. Except for the control group, AA model was established by Freund complete adjuvant. 12 days after modeling, control group and model group were given the same amount of normal saline, and other groups were given drugs by intragastric administration, once a day, for consecutive 16 days. Joint conditions of AA rats were evaluated by multiple arthritis and joint swelling. The level of NLRP3 protein in synovial tissues was detected by Western blot, and the expression and distribution of NLRP3 in synovial tissues were detected by immunohistochemical staining. Serum levels of interleukin-1β (IL-1β), IL-6 and tumor necrosis factor α (TNF-α) were detected by ELISA. Results Compared with the control group, multiple arthritis and joint swelling significantly increased in model group, while those significantly decreased in sinomenine treatment groups and Tripterygium wilfordii group. Decreased expression of NLRP3 protein in synovial tissue was observed, along with the significantly reduced levels of IL-1β, IL-6 and TNF-α in serum in sinoline treatment groups. Conclusion Sinolin can improve joint inflammation in AA rats by inhibiting NLRP3 and downstream inflammatory factors.
目的 探讨青藤碱对佐剂性关节炎（AA）大鼠的抗炎作用及含NOD样受体吡咯结构域蛋白3（NLRP3）的变化。方法 将Wistar大鼠随机分为对照组、AA模型组、（100、200、400）mg/kg青藤碱组和100 mg/kg雷公藤多苷组，每组10只。除对照组外，其余各组均采用弗氏完全佐剂建立AA模型。造模12天后，对照组和模型组给予等量生理盐水，其他各组灌胃给药，每日1次，连续16天。采用多关节炎评分和关节肿胀度评估AA大鼠的关节状况。采用蛋白质免疫印迹法检测滑膜组织中NLRP3蛋白水平，采用免疫组织化学染色法检测滑膜组织中NLRP3的表达及分布。采用酶联免疫吸附测定法检测血清白细胞介素-1β（IL-1β）、IL-6和肿瘤坏死因子α（TNF-α）水平。结果 与对照组比较，模型组多关节炎评分和关节肿胀度显著增加，而青藤碱治疗组和雷公藤多苷组显著降低。青藤碱治疗组滑膜组织中NLRP3蛋白表达降低，血清中IL-1β、IL-6和TNF-α水平显著降低。结论 青藤碱可通过抑制NLRP3及下游炎症因子改善AA大鼠的关节炎症。
