从 Rhynchostylis retusa (L.) Blume 的气生根中分离固氮菌的发生、鉴定和特性及其对植物生长促进活性的研究。
Occurrence, identification and characterization of diazotrophic bacteria from aerial roots of Rhynchostylis retusa (L.) Blume for plant growth-promoting activity.
机构信息
Microbial Biotechnology Laboratory, Biological Sciences and Technology Division, CSIR-North East Institute of Science and Technology, Jorhat, Assam, India.
Department of Applied Biology, University of Science and Technology, Baridua, Meghalaya, India.
出版信息
Arch Microbiol. 2023 Mar 22;205(4):131. doi: 10.1007/s00203-023-03458-3.
In this study, the diversity of diazotrophic bacteria of orchid Rhynchostylis retusa (L.) Blume and its potential application in plant growth promotion were evaluated. About 183 nitrogen-fixing bacteria were isolated to screen various plant growth-promoting traits viz. phosphate solubilization,IAA, siderophore, HCN, biofilm and ammonia production. Based on 16S rRNA gene sequencing analysis Achromobacter, Arthrobacter, Acinetobacter, Bacillus, Brevibacterium, Curtobacterium, Erwinia, Kosakonia, Lysinibacillus, Klebseilla, Microbacterium, Mixta, Pantoea, Pseudomonas and Stenotrophomonas isolates were selected and showed positive results for PGP traits. Overall, genus Pantoea, Brevibacterium, Achromobacter, Arthrobacter, Klebsiella, Mixta, Bacillus, and Pseudomonas had the most pronounced PGP characteristics and acetylene reduction among the screened isolates. BOX PCR fingerprinting analysis showed variation in polymorphic banding patterns among diazotrophic strains. PCR amplification of nifH gene and the presence of 37 kDa nitrogenase reductase enzyme band in western blot indicated presence of nitrogenase activity. Our study showed that orchid R. retusa diazotroph interaction helps orchid plant to fix nitrogen, essential nutrients, and control pathogen entry. To the best of our knowledge, this is the first report on characterization of diazotrophic bacterial community from aerial roots of R. retusa.
在这项研究中，评估了兰科植物 Rhynchostylis retusa（L.）Blume 的固氮细菌多样性及其在促进植物生长方面的潜在应用。分离出约 183 株固氮细菌，以筛选各种植物生长促进特性，如磷酸盐溶解、吲哚乙酸、铁载体、HCN、生物膜和氨产生。基于 16S rRNA 基因测序分析，选择了 Achromobacter、Arthrobacter、Acinetobacter、Bacillus、Brevibacterium、Curtobacterium、Erwinia、Kosakonia、Lysinibacillus、Klebseilla、Microbacterium、Mixta、Pantoea、Pseudomonas 和 Stenotrophomonas 分离株，并对其进行了 PGP 特性的阳性结果。总的来说，属 Pantoea、Brevibacterium、Achromobacter、Arthrobacter、Klebsiella、Mixta、Bacillus 和 Pseudomonas 具有最显著的 PGP 特性和在所筛选的分离物中乙炔还原能力。BOX PCR 指纹图谱分析显示固氮菌株之间存在多态性条带模式的变化。nifH 基因的 PCR 扩增和 Western blot 中 37 kDa 氮酶还原酶酶带的存在表明存在氮酶活性。我们的研究表明，兰花 R. retusa 与固氮菌的相互作用有助于兰花植物固定氮、必需营养物质和控制病原体进入。据我们所知，这是首次对 R. retusa 气生根固氮细菌群落进行特征描述的报道。
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