Departamento de Ciências Biomoleculares, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil; Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, United States.
Departamento de Ciências Biomoleculares, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil; Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA 02114, USA; Department of Dermatology, Harvard Medical School, Boston, MA 02115, USA.
J Inorg Biochem. 2023 Jun;243:112166. doi: 10.1016/j.jinorgbio.2023.112166. Epub 2023 Mar 3.
We have synthesized cis-[Ru(bpy)(NO-κN)L] and cis-[Ru(bpy)(NO-κO)L ] (bpy = 2,2'-bipyridine; k = indication of the coordinated center to Ruthenium; L = pyridine type ligand) by reacting cis-[Ru(bpy)(HO)L] with sodium nitrite or conducting basic cis-[Ru(bpy)NO(L)] hydrolysis. Photolysis at the metal-ligand charge transfer band (MLCT) of the isomers yielded nitric oxide (NO) as determined by NO measurement. The NO photorelease rates obtained upon 447 nm laser irradiation of the ruthenium complexes showed that cis-[Ru(bpy)(NO-κO)L] released NO three times faster than cis-[Ru(bpy)(NO-κN)L]. We investigated endothelium-dependent vasodilation induced by cis-[Ru(bpy)(4-pic)(NO-κN)] and cis-[Ru(bpy)(4-pic)(NO-κO)] (4-pic = 4-picoline) in isolated 3 mm aortic rings precontracted with L-phenylephrine. Maximum vasodilation was achieved under 447 nm laser irradiation of 0.5 μMol.L ruthenium complexes for 100 s.
我们通过用亚硝酸钠与 cis-[Ru(bpy)(HO)L]反应或进行碱性 cis-[Ru(bpy)NO(L)]水解，合成了 cis-[Ru(bpy)(NO-κN)L]和 cis-[Ru(bpy)(NO-κO)L]（bpy = 2,2'-联吡啶；k = 表示配位中心至钌；L = 吡啶型配体）。异构体的金属-配体电荷转移带（MLCT）光解生成了一氧化氮（NO），通过 NO 测量确定。在 447nm 激光辐照钌配合物时获得的 NO 光释放速率表明，cis-[Ru(bpy)(NO-κO)L]的 NO 释放速度比 cis-[Ru(bpy)(NO-κN)L]快三倍。我们研究了 cis-[Ru(bpy)(4-pic)(NO-κN)]和 cis-[Ru(bpy)(4-pic)(NO-κO)]（4-pic = 4-吡啶）诱导的离体 3mm 主动脉环内皮依赖性血管舒张，4-pic = 4-吡啶）在预先用 L-苯肾上腺素收缩的离体 3mm 主动脉环中。在 447nm 激光照射下，最大血管舒张发生在 0.5μMol.L 的钌络合物辐照 100s 时。
