前列腺特异性抗原在细胞外微囊泡中的反应性对商业免疫分析的影响。

PSA reactivity in extracellular microvesicles to commercial immunoassays.

机构信息

Service of Biochemistry, Clínica Universidad de Navarra, Av. Pío XII 36, 31008 Pamplona, Spain.

Oncology Department, Clínica Universidad de Navarra, Av. Pío XII 36, 31008 Pamplona, Spain; IdiSNA, Navarra Institute for Health Research, Calle Irunlarrea 3, 31008 Pamplona, Spain.

出版信息

Clin Chim Acta. 2023 Mar 15;543:117303. doi: 10.1016/j.cca.2023.117303. Epub 2023 Mar 21.

DOI:10.1016/j.cca.2023.117303

PMID:36948237

Abstract

AIMS

Characterization of PSA in extracellular microvesicles (EVs) and its reactivity to commercial methods.

MATERIALS AND METHODS

EVs derived from serum of 47 prostate cancer (PCa) patients, 27 benign prostatic hyperplasia (BPH) patients and 42 healthy controls were analyzed. EVs isolation and quantification of PSA immunoreactive to total (ev-T-PSA) or free (ev-F-PSA) PSA immunoassays, were performed using commercial assays. PSA in CD81+ or CD63+ EVs was determined directly in serum by an immunocapture-ELISA (IC-ELISA).

RESULTS

Ev-T-PSA immunoreactive to Elecsys assay was detected in all samples. Median T-PSA ev/srm ratio was 2.20 % (Q1-Q3: 0.80-4.00 %), although in some samples this ratio reached 59 %. T-PSA ev/srm ratio was higher in those samples with serum T-PSA below 4 µg/L than in those exceeding that cut-off (p < 0.001). T-PSA ev/srm ratio was lower in PCa patients compared to healthy controls and BPH patients (p < 0.001). Elecsys immunoassays detected higher concentrations of ev-T-PSA and ev-F-PSA than Immulite (p < 0.001). PSA was detected by IC-ELISA more intensely in CD81+ EVs than in CD63+ EVs, and ev-T-PSA correlated with PSA+ CD63+ (p < 0.001) but not with PSA+ CD81+.

CONCLUSION

EVs-bound PSA is another form of circulating PSA whose measurement could be easily performed in clinical laboratories by automated immunoassays.

摘要

目的

研究细胞外微泡（EVs）中 PSA 的特征及其对商业方法的反应。

材料与方法

分析了 47 例前列腺癌（PCa）患者、27 例良性前列腺增生（BPH）患者和 42 例健康对照者的血清来源 EVs。采用商业试剂盒检测 EVs 分离和总 PSA（ev-T-PSA）或游离 PSA（ev-F-PSA）免疫分析中 PSA 的免疫反应性。使用免疫捕获 ELISA（IC-ELISA）直接在血清中测定 CD81+或 CD63+EVs 中的 PSA。

结果

所有样本中均检测到 Elecsys 检测法的 ev-T-PSA 免疫反应性。中位 ev/srm T-PSA 比值为 2.20%（Q1-Q3：0.80-4.00%），但在某些样本中该比值可达 59%。血清 T-PSA 低于 4μg/L 的样本中，T-PSA ev/srm 比值高于超过该截止值的样本（p<0.001）。PCa 患者的 T-PSA ev/srm 比值低于健康对照者和 BPH 患者（p<0.001）。Elecsys 免疫分析法检测到的 ev-T-PSA 和 ev-F-PSA 浓度高于 Immulite（p<0.001）。IC-ELISA 检测到 CD81+EVs 中的 PSA 比 CD63+EVs 更强烈，ev-T-PSA 与 PSA+CD63+相关（p<0.001），但与 PSA+CD81+无关。