Cadi Ayyad University, Faculty of Sciences Semlalia, 2390, 40000, Marrakech, Morocco; Institute of Biological Sciences, ISSB, Faculty of medical sciences (FMS), Mohammed VI Polytechnic University (UM6P), Ben Guerir, 43150, Morocco.
Institute of Biological Sciences, ISSB, Faculty of medical sciences (FMS), Mohammed VI Polytechnic University (UM6P), Ben Guerir, 43150, Morocco.
Int J Pharm. 2023 Apr 25;637:122861. doi: 10.1016/j.ijpharm.2023.122861. Epub 2023 Mar 21.
This work aims to develop an injectable and antibacterial composite cement for bone substitution and prevention/treatment of bone infections. This cement is composed of calcium phosphate, calcium carbonate, bioactive glass, sodium alginate, and ciprofloxacin. The effect of ciprofloxacin on the microstructure, chemical composition, setting properties, cohesion, injectability, and compressive strength was investigated. The in vitro drug release kinetics and the antibacterial activity of ciprofloxacin-loaded composites against staphylococcus aureus and Escherichia coli pathogens were investigated. XRD and FTIR analysis demonstrated that the formulated cements are composed of a nanocrystalline carbonated apatite analogous to the mineral part of the bone. The evaluation of the composite cement's properties revealed that the incorporation of 3 and 9 wt% of ciprofloxacin affects the microstructural and physicochemical properties of the cement, resulting in a prolonged setting time, and a slight decrease in injectability and compressive strength. The in vitro drug release study revealed sustained release profiles over 18 days. The amounts of ciprofloxacin released per day (0.2 -15.2 mg/L) depend on the cement composition and the amount of ciprofloxacin incorporated. The antibacterial activity of ciprofloxacin-loaded cement composites attested to their effectiveness to inhibit the growth of Staphylococcus aureus and Escherichia coli.
本工作旨在开发一种可注射的抗菌复合骨水泥，用于骨替代和预防/治疗骨感染。该水泥由磷酸钙、碳酸钙、生物活性玻璃、海藻酸钠和环丙沙星组成。研究了环丙沙星对微观结构、化学成分、凝固性能、内聚性、可注射性和抗压强度的影响。研究了载药复合材料对金黄色葡萄球菌和大肠杆菌病原体的体外药物释放动力学和抗菌活性。XRD 和 FTIR 分析表明，所配制的水泥由类似于骨矿物部分的纳米晶碳酸磷灰石组成。对复合水泥性能的评价表明，掺入 3%和 9%wt%的环丙沙星会影响水泥的微观结构和物理化学性质，导致凝固时间延长，可注射性和抗压强度略有降低。体外药物释放研究显示，18 天内呈现持续释放曲线。每天释放的环丙沙星量（0.2-15.2mg/L）取决于水泥的组成和掺入的环丙沙星量。载药水泥复合材料的抗菌活性证明了其抑制金黄色葡萄球菌和大肠杆菌生长的有效性。
