Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, Schöpfstrasse 41/R311, 6020, Innsbruck, Austria.
School of Natural Sciences and Medicine Tbilisi, Ilia State University, Tbilisi, Georgia.
Respir Res. 2023 Mar 22;24(1):88. doi: 10.1186/s12931-023-02397-3.
New SARS-CoV-2 variants of concern (VOCs) and waning immunity illustrate that quick and easy-to-use agents are needed to prevent infection. To protect from viral transmission and subsequent inflammatory reactions, we applied GlyperA™, a novel antimicrobial formulation that can be used as mouth gargling solution or as nasal spray, to highly differentiated human airway epithelia prior infection with Omicron VOCs BA.1 and BA.2. This formulation fully protected polarized human epithelium cultured in air-liquid interphase (ALI) from SARS-CoV-2-mediated tissue destruction and infection upon single application up to two days post infection. Moreover, inflammatory reactions induced by the Omicron VOCs were significantly lowered in tissue equivalents either pre-treated with the GlyperA™ solution, or even when added simultaneously. Thus, the GlyperA™ formulation significantly shielded epithelial integrity, successfully blocked infection with Omicron and release of viral particles, and decreased intracellular complement C3 activation within human airway epithelial cell cultures. Crucially, our in vitro data imply that GlyperA™ may be a simple tool to prevent from SARS-CoV-2 infection independent on the circulating variant via both, mouth and nose.
新型严重急性呼吸综合征冠状病毒 2 变异株（VOCs）和免疫功能下降表明，需要快速、易于使用的药物来预防感染。为了防止病毒传播和随后的炎症反应，我们在感染奥密克戎变异株 BA.1 和 BA.2 之前，将一种新型的抗菌配方 GlyperA™应用于高度分化的人呼吸道上皮细胞。该配方在单剂量应用后，可完全保护在气液界面（ALI）培养的极化人上皮细胞免受 SARS-CoV-2 介导的组织破坏和感染，甚至在感染后两天内也有保护作用。此外，用 GlyperA™溶液预处理或同时添加时，奥密克戎 VOCs 诱导的炎症反应明显降低。因此，GlyperA™配方显著保护了上皮细胞的完整性，成功阻断了奥密克戎的感染和病毒颗粒的释放，并减少了人呼吸道上皮细胞培养物中细胞内补体 C3 的激活。至关重要的是，我们的体外数据表明，GlyperA™可能是一种通过口腔和鼻腔预防 SARS-CoV-2 感染的简单工具，与循环变异株无关。
