Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Jiangsu Provincial Key Lab for the Chemistry and Utilization of Agro-Forest Biomass, College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China.
Bioprocess Biosyst Eng. 2023 Jun;46(6):829-837. doi: 10.1007/s00449-023-02865-6. Epub 2023 Mar 23.
In this study, the synthesis of xylonic acid from xylose by Gluconobacter oxydans NL71 has been investigated. According to the relationship between oxygen transfer rate and oxygen uptake rate, three different kinetic models of product formation were established and the nonlinear fitting was carried out. The results showed that G. oxydans has critical dissolved oxygen under different strain concentrations, and the relationship between respiration intensity and dissolved oxygen conformed to the Monod equation [Formula: see text]. The maximum reaction rate per unit cell mass and the theoretical maximum specific productivity of G. oxydans obtained by the kinetic model are 0.042 mol/L/h and 6.97 g/g/h, respectively. These results will assist in determining the best balance between the airflow rate and cell concentration in the reaction and improve the production efficiency of xylonic acid.
本研究以氧化葡萄糖酸杆菌 NL71 为出发菌株，对木糖转化生成戊二酸的过程进行了研究。根据氧传递速率与需氧速率的关系，建立了三种不同的产物形成动力学模型，并进行了非线性拟合。结果表明，氧化葡萄糖酸杆菌在不同菌浓下存在临界溶解氧，呼吸强度与溶解氧的关系符合 Monod 方程。通过动力学模型得到的单位细胞质量的最大比反应速率和理论最大比产率分别为 0.042 mol/L/h 和 6.97 g/g/h。这些结果将有助于确定反应中空气流量和细胞浓度的最佳平衡，提高戊二酸的生产效率。
