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新型酵母中间型假丝酵母 FL023 利用木质纤维素水解物和木糖生产单细胞蛋白和木糖醇。

Single-cell Protein and Xylitol Production by a Novel Yeast Strain Candida intermedia FL023 from Lignocellulosic Hydrolysates and Xylose.

机构信息

State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.

Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture), Biomass Energy Technology Research Centre, Biogas Institute of Ministry of Agriculture, Chengdu, 610041, Sichuan, People's Republic of China.

出版信息

Appl Biochem Biotechnol. 2018 May;185(1):163-178. doi: 10.1007/s12010-017-2644-8. Epub 2017 Nov 2.

Abstract

Yeasts are good candidates to utilize the hydrolysates of lignocellulose, the most abundant bioresource, for bioproducts. This study aimed to evaluate the efficiencies of single-cell protein (SCP) and xylitol production by a novel yeast strain, Candida intermedia FL023, from lignocellulosic hydrolysates and xylose. This strain efficiently assimilated hexose, pentose, and cellubiose for cell mass production with the crude protein content of 484.2 g kg dry cell mass. SCP was produced by strain FL023 using corncob hydrolysate and urea as the carbon and nitrogen sources with the dry cell mass productivity 0.86 g L h and the yield of 0.40 g g sugar. SCP was also produced using NaOH-pretreated Miscanthus sinensis straw and corn steep liquor as the carbon and nitrogen sources through simultaneous saccharification and fermentation with the dry cell productivity of 0.23 g L h and yield of 0.17 g g straw. C. intermedia FL023 was tolerant to 0.5 g L furfural, acetic acid, and syringaldehyde in xylitol fermentation and produced 45.7 g L xylitol from xylose with the productivity of 0.38 g L h and the yield of 0.57 g g xylose. This study provides feasible methods for feed and food additive production from the abundant lignocellulosic bioresources.

摘要

酵母是利用木质纤维素这种最丰富的生物资源的水解产物生产生物制品的理想候选物。本研究旨在评估新型酵母中间假丝酵母 FL023 利用木质纤维素水解物和木糖生产单细胞蛋白 (SCP) 和木糖醇的效率。该菌株能够有效地同化己糖、戊糖和纤维二糖来生产细胞物质,其粗蛋白含量为 484.2 g kg 干细胞质量。FL023 菌株使用玉米芯水解物和尿素作为碳源和氮源生产 SCP,其干细胞质量生产率为 0.86 g L h,糖得率为 0.40 g g 糖。同时糖化发酵使用预处理后的芒草和玉米浆作为碳源和氮源也可以生产 SCP,其干细胞生产率为 0.23 g L h,糖得率为 0.17 g g 秸秆。中间假丝酵母 FL023 能够耐受 0.5 g L 糠醛、乙酸和丁香醛在木糖醇发酵中,能够从木糖生产 45.7 g L 木糖醇,生产率为 0.38 g L h,木糖得率为 0.57 g g 木糖。本研究为利用丰富的木质纤维素生物资源生产饲料和食品添加剂提供了可行的方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2018/5937888/12234bbfacc3/12010_2017_2644_Fig1_HTML.jpg

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