在分枝杆菌感染期间检测过氧化物酶体蛋白。

Detection of Peroxisomal Proteins During Mycobacterial Infection.

机构信息

School of Biotechnology, KIIT Deemed to be University, Bhubaneswar, Odisha, India.

Institute of Anatomy and Cell Biology, Julius-Maximilians-University Würzburg, Würzburg, Germany.

出版信息

Methods Mol Biol. 2023;2643:123-134. doi: 10.1007/978-1-0716-3048-8_9.

DOI:10.1007/978-1-0716-3048-8_9

PMID:36952182

Abstract

Peroxisomes are ubiquitous organelles with essential roles in lipid and reactive oxygen species (ROS) metabolism. They are involved in modulating the immune responses during microbial infection, thus having major impact on several bacterial and viral infectious diseases including tuberculosis. Intracellular pathogens such as Mycobacterium tuberculosis (M. tb) employ various strategies to suppress the host oxidative stress mechanisms to avoid killing by the host. Peroxisome-mediated ROS balance is crucial for innate immune responses to M. tb. Therefore, peroxisomes represent promising targets for host-directed therapeutics to tuberculosis. Here, we present protocols used in our laboratory for the culture of M. tb and detection of peroxisomal proteins in M. tb infected macrophages.

摘要

过氧化物酶体是普遍存在的细胞器，在脂质和活性氧（ROS）代谢中具有重要作用。它们参与调节微生物感染期间的免疫反应，因此对包括结核病在内的几种细菌和病毒感染性疾病有重大影响。细胞内病原体，如结核分枝杆菌（M. tb），采用各种策略来抑制宿主的氧化应激机制，以避免被宿主杀死。过氧化物酶体介导的 ROS 平衡对于对 M. tb 的先天免疫反应至关重要。因此，过氧化物酶体是结核病宿主导向治疗的有前途的靶点。在这里，我们展示了我们实验室中用于培养 M. tb 和检测感染巨噬细胞中过氧化物酶体蛋白的方案。