微核试验是否适合用于生物监测儿童 X 射线暴露？系统评价与荟萃分析。
Is micronucleus assay a suitable method for biomonitoring children exposed to X-ray? A systematic review with meta-analysis.
机构信息
Department of Biosciences, Institute of Health and Society, Federal University of São Paulo, UNIFESP, Santos, Brazil.
Department of Diagnosis and Surgery, School of Dentistry, Sao Paulo State University, UNESP, Araçatuba, Brazil.
出版信息
Int J Radiat Biol. 2023;99(10):1522-1530. doi: 10.1080/09553002.2023.2194405. Epub 2023 Mar 31.
PURPOSE
The aim of this study was to evaluate if the micronucleus test using oral epithelial cells is a suitable biomarker for biomonitoring children exposed to X-ray.
MATERIAL AND METHODS
A search was performed through the electronic databases PubMed/Medline, Scopus, and Web of Science, all studies published up to February 2022 that examined the relationship between exposure of children to radiographic examinations and micronucleus.
RESULTS
A total of 17 full-text manuscripts were screened for eligibility. Only two studies found a difference in micronucleus labeling. On the other hand, all studies showed that X-ray was able to induce cellular death in oral mucosa cells. Following the parameters of the Effective Practices in Public Health Project (EPHPP), five manuscripts reached moderate and strong scores, and four studies were categorized as weak at final rating. In the meta-analysis, statistically significant difference was detected in micronucleated cells in children before and after radiographic examinations (SMD = 0.96, 95% CI, 0.07-1.84, = .04), with τ=1.09; χ=53.37, and < .001.
CONCLUSION
Radiographic examinations in children can cause genotoxic and cytotoxic damage in the oral epithelium with a large effect size.
目的
本研究旨在评估口腔上皮细胞微核试验是否可作为儿童 X 射线暴露生物监测的合适生物标志物。
材料与方法
通过电子数据库 PubMed/Medline、Scopus 和 Web of Science 进行检索，检索截至 2022 年 2 月所有研究儿童放射检查暴露与微核之间关系的文章。
结果
共筛选出 17 篇全文进行资格审查。只有两项研究发现微核标记有差异。另一方面，所有研究均表明 X 射线能够诱导口腔黏膜细胞死亡。根据有效公共卫生实践项目（EPHPP）的参数，5 篇文章获得了中等和强评分，4 项研究最终被评为弱。在荟萃分析中，检测到儿童放射检查前后微核细胞存在统计学显著差异（SMD=0.96，95%CI，0.07-1.84， = .04），τ=1.09；χ=53.37，且 < .001。
结论
儿童放射检查可导致口腔上皮的遗传毒性和细胞毒性损伤，其效应量较大。
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