Drumheller P D, Van Wie B J, Petersen J N, Oxford R J, Schneider G W
Chemical Engineering Department, Washington State University, Pullman 99164-2710.
J Biomech Eng. 1987 Nov;109(4):324-9. doi: 10.1115/1.3138688.
A COBE blood cell centrifuge, model 2997 with a single stage channel, was modified to allow computer controlled sampling, and to allow recycle of red blood cells (RBCs) and plasma streams using bovine whole blood. The effects of recycle of the packed RBC and plasma product streams, and of the centrifuge RPM on platelet and white blood cell (WBC) separation efficiencies were quantified using a central composite factorial experimental design. These data were then fit using second order models. Both the model for the WBC separation efficiency and the model for the platelet separation efficiency predict that RPM has the greatest effect on separation efficiency and that RBC and plasma recycle have detrimental effects at moderate to low RPM, but have negligible impact on separation efficiency at high RPM.
一台COBE 2997型单级通道血细胞离心机进行了改装，以实现计算机控制采样，并能使用牛全血对红细胞（RBC）和血浆流进行再循环。采用中心复合因子实验设计，对压实红细胞和血浆产物流再循环以及离心机每分钟转速（RPM）对血小板和白细胞（WBC）分离效率的影响进行了量化。然后使用二阶模型对这些数据进行拟合。白细胞分离效率模型和血小板分离效率模型均预测，转速对分离效率影响最大，在中低转速下，红细胞和血浆再循环会产生不利影响，但在高转速下对分离效率的影响可忽略不计。
