Sasaki K, LaMorte W W, Nickerson C J, Fuller R M, Chobanian A V, Menzoian J O
Department of Surgery, Boston University Medical Center, MA 02118.
J Surg Res. 1987 Dec;43(6):565-70. doi: 10.1016/0022-4804(87)90132-6.
Increased arterial permeability to [125I]albumin is one of the earliest abnormalities found in the cholesterol-fed rabbit model for atherosclerosis and precedes morphologic changes in the arterial wall. Since propranolol is reported to retard atherogenesis in this model, the current study was undertaken to study the effect of propranolol on arterial permeability to albumin in cholesterol-fed rabbits. Carotid permeability to [125I]albumin was measured after 1 week of (a) a control diet containing only trace cholesterol (N = 9), (b) a 1.5% cholesterol diet (N = 11), or (c) a 1.5% cholesterol diet plus intraperitoneal propranolol 5 mg/kg/day (N = 19). The carotid artery was cannulated proximally and distally and peristaltically perfused with oxygenated Modified Eagle's Medium to which [125I]albumin had been added. The permeability of the carotid artery to albumin was estimated by measuring 125I activity in peripheral venous samples obtained after 0, 15, 30, 60, 120, 180, and 240 min of carotid perfusion. 125I-radioactivity of the perfused carotid artery was also counted at the end of the experiment to measure 125I retained in the arterial wall. The transfer of [125I]albumin across the arterial wall to the venous circulation was greater in the untreated cholesterol-fed group than in controls (P less than 0.05), but cholesterol-fed animals treated with propranolol did not differ significantly from controls. 125I-albumin in the arterial wall after 240 min of perfusion was decreased in both untreated cholesterol-fed animals (1444 dpm/cm +/- 350) and in propranolol-treated animals (1629 +/- 309) when these were compared to controls (3909 +/- 693, P less than .05).(ABSTRACT TRUNCATED AT 250 WORDS)
动脉对[125I]白蛋白通透性增加是在喂胆固醇兔动脉粥样硬化模型中最早发现的异常之一，且早于动脉壁的形态学改变。由于据报道普萘洛尔可延缓该模型中的动脉粥样硬化形成，因此进行了本研究以探讨普萘洛尔对喂胆固醇兔动脉白蛋白通透性的影响。在以下三种情况喂养1周后测量颈动脉对[125I]白蛋白的通透性：(a)仅含微量胆固醇的对照饮食（N = 9），(b)1.5%胆固醇饮食（N = 11），或(c)1.5%胆固醇饮食加腹腔注射普萘洛尔5 mg/kg/天（N = 19）。在颈动脉近端和远端插管，并用添加了[125I]白蛋白的充氧改良伊格尔培养基进行蠕动灌注。通过测量颈动脉灌注0、15、30、60、120、180和240分钟后外周静脉样本中的125I活性来估计颈动脉对白蛋白的通透性。实验结束时还对灌注的颈动脉的125I放射性进行计数，以测量保留在动脉壁中的125I。未治疗的喂胆固醇组中，[125I]白蛋白穿过动脉壁进入静脉循环的量比对照组更大（P < 0.05），但用普萘洛尔治疗的喂胆固醇动物与对照组无显著差异。与对照组（3909 ± 693，P < 0.05）相比，灌注240分钟后，未治疗的喂胆固醇动物（1444 dpm/cm ± 350）和普萘洛尔治疗的动物（1629 ± 309）动脉壁中的125I-白蛋白均减少。（摘要截短至250字）
