Key Laboratory of Bio-resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, Sichuan, PR China.
College of Pharmacy, Chengdu Medical College, Chengdu, Sichuan, China.
J Environ Manage. 2023 Jul 1;337:117723. doi: 10.1016/j.jenvman.2023.117723. Epub 2023 Mar 21.
Application of plant growth-promoting rhizobacteria plays a vital role in enhancing phytoremediation efficiency. In this study, multiple approaches were employed to investigate the underlying mechanisms of Burkholderia sp. SRB-1 (SRB-1) on elevating Cd uptake and accumulation. Inoculation experiment indicated that SRB-1 could facilitate plant growth and Cd tolerance, as evidenced by the enhanced plant biomass and antioxidative enzymes activities. Cd content in plant shoots and roots increased about 36.56%-39.66% and 25.97%-130.47% assisted with SRB-1 when compared with control. Transcriptomics analysis revealed that SRB-1 upregulated expression of amiE, AAO1-2 and GA2-ox related to auxin and gibberellin biosynthesis in roots. Auxin and gibberellin, as hormone signals, regulated plant Cd tolerance and growth through activating hormone signal transduction pathways, which might also contribute to 67.94% increase of dry weight. The higher expression levels of ATP-binding cassette transporter subfamilies (ABCB, ABCC, ABCD and ABCG) in Chrysopogon zizanioides roots contributed to higher Cd uptake in Cd15 B (323.83 mg kg) than Cd15 (136.28 mg kg). Further, SRB-1 facilitated Cd migration from roots to shoots via upregulating the expression of Nramp, ZIP and HMA families. Our integrative analysis provided a molecular-scale perspective on Burkholderia sp. SRB-1 contributing to C. zizanioides performance.
植物促生根际细菌的应用在提高植物修复效率方面起着至关重要的作用。本研究采用多种方法研究了伯克霍尔德氏菌（SRB-1）提高 Cd 吸收和积累的潜在机制。接种实验表明，SRB-1 可以促进植物生长和 Cd 耐受，表现在植物生物量和抗氧化酶活性的增强。与对照相比，SRB-1 可使植物地上部和根部的 Cd 含量分别增加 36.56%-39.66%和 25.97%-130.47%。转录组学分析表明，SRB-1 上调了根中与生长素和赤霉素生物合成相关的 amiE、AAO1-2 和 GA2-ox 的表达。作为激素信号，生长素和赤霉素通过激活激素信号转导途径调节植物 Cd 耐受和生长，这也可能导致干重增加 67.94%。香根草根中 ABC 转运蛋白亚家族（ABCB、ABCC、ABCD 和 ABCG）的高表达水平有助于 Cd15B（323.83mgkg）中 Cd 的吸收高于 Cd15（136.28mgkg）。此外，SRB-1 通过上调 Nramp、ZIP 和 HMA 家族的表达促进 Cd 从根部向地上部的迁移。我们的综合分析为伯克霍尔德氏菌（SRB-1）促进香根草性能提供了一个分子水平的视角。
