Bridges Blake O, Tice Abigail L, Laudato Joseph A, Gordon Bradley S, Steiner Jennifer L
Department of Nutrition and Integrative Physiology, Florida State University, 600 W. College Avenue, Tallahassee, FL, 32306, USA.
Department of Nutrition and Integrative Physiology, Florida State University, 600 W. College Avenue, Tallahassee, FL, 32306, USA; Institute of Sports Sciences and Medicine, Florida State University, 600 W. College Avenue, Tallahassee, FL, 32306, USA.
Mol Cell Endocrinol. 2023 May 1;566-567:111914. doi: 10.1016/j.mce.2023.111914. Epub 2023 Mar 22.
To determine whether alcohol consumed within the meal influences the feeding induced increase in mTORC1 signaling.
Alcohol provided in the liquid diet was consumed by alcohol naïve, fasted, C57BL/6Hsd female mice and gastrocnemius was collected 1hr after the refeeding. Subsequent experiments determined the extent to which changes in mTORC1 signaling persisted across the day.
Compared with control mice, protein synthesis, mTORC1 (Ser2448), 4EBP1 (Ser65), S6K1 (Thr389), rpS6 (Ser240/244), Akt (Thr308), and ULK1 (Ser757) were lower in EtOH. Similar suppressive patterns were observed in the hours following consumption of alcohol containing food throughout the dark cycle. Higher peak blood alcohol concentrations induced by intraperitoneal injection of alcohol extended the time and magnitude of mTORC1 pathway suppression.
Alcohol administered as part of the meal results in lower skeletal muscle mTORC1 signaling while subsequent models show that alcohol may influence this pathway across the day.
确定进餐时摄入的酒精是否会影响进食诱导的mTORC1信号通路增加。
初次接触酒精、禁食的C57BL/6Hsd雌性小鼠饮用含酒精的流质饮食，再喂食1小时后采集腓肠肌。后续实验确定mTORC1信号通路变化在一天中持续的程度。
与对照小鼠相比，乙醇组的蛋白质合成、mTORC1（Ser2448）、4EBP1（Ser65）、S6K1（Thr389）、rpS6（Ser240/244）、Akt（Thr308）和ULK1（Ser757）水平较低。在整个黑暗周期中，食用含酒精食物后的数小时内观察到类似的抑制模式。腹腔注射酒精诱导的更高的血酒精浓度峰值延长了mTORC1信号通路抑制的时间和程度。
进餐时摄入酒精会导致骨骼肌mTORC1信号通路降低，而后续模型表明酒精可能在一天中影响该信号通路。
