Quality Estimates for 3D Protein Models.

Protein structure modeling is one of the most advanced and complex processes in computational biology. One of the major problems for the protein structure prediction field has been how to estimate the accuracy of the predicted 3D models, on both a local and global level, in the absence of known structures. We must be able to accurately measure the confidence that we have in the quality predicted 3D models of proteins for them to become widely adopted by the general bioscience community. To address this major issue, it was necessary to develop new model quality assessment (MQA) methods and integrate them into our pipelines for building 3D protein models. Our MQA method, called ModFOLD, has been ranked as one of the most accurate MQA tools in independent blind evaluations. This chapter discusses model quality assessment in the protein modeling field, demonstrating both its strengths and limitations. We also present some of the best methods according to independent benchmarking data, which has been gathered in recent years.