InnoGenomics Technologies, LLC, 2000 Lakeshore Drive, #5016, New Orleans, Louisiana, 70148, USA.
J Forensic Sci. 2023 May;68(3):1020-1035. doi: 10.1111/1556-4029.15239. Epub 2023 Mar 23.
Forensic casework samples often include human hairs, teeth, and bones. Hairs with roots are routinely processed for DNA analysis, while rootless hairs are either not tested or processed using mitochondrial DNA. Bones and teeth are submitted for human remains identifications for missing persons and mass disaster cases. DNA extraction from these low templates and degraded samples is challenging. The new InnoXtract DNA extraction method utilizes magnetic beads that are optimized to bind small DNA fragments, as small as 100 base pairs, to purify high-yield DNA from compromised samples. This validation study evaluates InnoXtract's ability to obtain amplifiable DNA from samples such as rootless hairs and skeletal remains. Studies performed include sensitivity, stability, repeatability, reproducibility, non-probative samples, and comparison to standard organic extractions. Sensitivity studies demonstrate average yield recoveries ranging from 53% to 100% and 73% to 85% for the InnoXtract hair and bone methods, respectively. Studies demonstrate consistent results across a range of sample types, such as insulted and un-insulted bone and teeth, as well as hair shafts from donors of various ages, gender, race, and hair characteristics. The InnoXtract bone method outperformed organic extraction. The method was successfully automated on a MagMAX™ Express-96, with recoveries over 70% relative to the manual version. InnoXtract has the potential as an automated high-throughput, high-yield bone extraction method with 6 h of total extraction time for up to 96 samples. The validation study results demonstrate that the InnoXtract kits produce high-yield and high-quality DNA from compromised bone, teeth, and hair shaft samples.
法医案例工作样本通常包括人类毛发、牙齿和骨骼。带根毛发通常经过 DNA 分析处理，而无根毛发则要么未经测试，要么使用线粒体 DNA 进行处理。骨骼和牙齿被提交用于失踪人员和大规模灾难案件的人类遗骸识别。从这些低模板和降解样本中提取 DNA 具有挑战性。新型 InnoXtract DNA 提取方法利用了优化的磁性珠，这些磁性珠可以结合小 DNA 片段，从受损样本中纯化高产量 DNA。这项验证研究评估了 InnoXtract 从无根毛发和骨骼遗骸等样本中获取可扩增 DNA 的能力。进行的研究包括灵敏度、稳定性、重复性、再现性、非证明性样本以及与标准有机提取的比较。灵敏度研究表明，InnoXtract 毛发和骨骼方法的平均产量回收率分别为 53%至 100%和 73%至 85%。研究表明，在各种样本类型（如受侮辱和未受侮辱的骨骼和牙齿，以及来自不同年龄、性别、种族和毛发特征的供体的毛发轴）中，结果一致。InnoXtract 骨骼方法优于有机提取。该方法已成功在 MagMAX™ Express-96 上实现自动化，与手动版本相比，回收率超过 70%。InnoXtract 有可能成为一种自动化高通量、高产量的骨骼提取方法，总提取时间为 6 小时，最多可处理 96 个样本。验证研究结果表明，InnoXtract 试剂盒可从受损的骨骼、牙齿和毛发轴样本中产生高产和高质量的 DNA。
