Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba City, Japan.
Department of Orthopaedic Surgery, Nagareyama Central Hospital, Nagareyama, Japan.
J Orthop Res. 2023 Nov;41(11):2359-2366. doi: 10.1002/jor.25560. Epub 2023 Apr 4.
The rat mono-iodoacetate (MIA) arthritis model has been used in studies on the hip, knee, and ankle joints. Few studies have explored its utility in shoulder arthritis research, and none have evaluated the effects of time and different MIA doses on arthritis progression. Therefore, we developed a rat MIA shoulder arthritis model to evaluate articular changes through radiological and histological analyses. Sprague-Dawley rats (n = 108) were equally divided into groups that were intra-articularly injected with 0.5 mg of MIA (in 50 µL of purified water), 2.0 mg of MIA (in 50 µL of purified water), or purified water (50 µL; sham group). Throughout the study period, 18 rats (six per group) were evaluated by computed tomography and assessed using the Larsen's classification system; 90 rats were further evaluated histologically using the Osteoarthritis Research Society International scoring system. Computed tomography revealed that the groups injected with MIA developed arthritis and osteophytes 14 days after injection, which progressed temporally. The Larsen's grades worsened over time; at all time points, the scores were higher in the group injected with 2.0 mg of MIA than in the group injected with 0.5 mg of MIA. Furthermore, concurrent with the worsening Larsen's grades, the Osteoarthritis Research Society International scores also significantly increased over time; at all time points, they were higher in the group injected with 2.0 mg of MIA than in the group injected with 0.5 mg of MIA. Our rat MIA shoulder arthritis model revealed radiologically and histologically confirmed temporal and MIA dose-dependent arthritic changes.
鼠碘乙酸盐（MIA）关节炎模型已被用于髋关节、膝关节和踝关节的研究。很少有研究探讨其在肩关节关节炎研究中的应用，也没有研究评估时间和不同 MIA 剂量对关节炎进展的影响。因此，我们建立了一个大鼠 MIA 肩关节关节炎模型，通过影像学和组织学分析评估关节变化。将 108 只 Sprague-Dawley 大鼠（n=108）等分为四组，分别向关节内注射 0.5mg MIA（50μL 去离子水）、2.0mg MIA（50μL 去离子水）或去离子水（50μL；假手术组）。在整个研究期间，18 只大鼠（每组 6 只）通过计算机断层扫描进行评估，并使用 Larsen 分类系统进行评估；90 只大鼠进一步通过骨关节炎研究协会国际评分系统进行组织学评估。计算机断层扫描显示，注射 MIA 的大鼠在注射后 14 天出现关节炎和骨赘，且随时间推移逐渐进展。Larsen 评分随时间恶化；在所有时间点，2.0mg MIA 组的评分均高于 0.5mg MIA 组。此外，与 Larsen 评分恶化同时发生的是，骨关节炎研究协会国际评分系统也随时间显著增加；在所有时间点，2.0mg MIA 组的评分均高于 0.5mg MIA 组。我们的大鼠 MIA 肩关节关节炎模型显示出影像学和组织学上证实的时间依赖性和 MIA 剂量依赖性关节炎变化。
