环状肉芽肿和结节病中 MMP-9 和 pSTAT1 的免疫组织化学特征：62 例对比研究。

Immunohistochemical Features of MMP-9 and pSTAT1 in Granuloma Annulare and Sarcoidosis: A Comparative Study of 62 Cases.

机构信息

Department of Dermatology, General Hospital of Northern Theater Command, No. 83 Wenhua Road, Shenhe District, 110016 Shenyang, China.

College of Medicine and Biological Information Engineering, Northeastern University, No. 3-11, Wenhua Road, Heping District, 110819 Shenyang, China.

出版信息

J Immunol Res. 2023 Mar 14;2023:4098459. doi: 10.1155/2023/4098459. eCollection 2023.

DOI:10.1155/2023/4098459

PMID:36959923

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10030222/

Abstract

BACKGROUND

Granuloma annulare (GA) and sarcoidosis are granulomatous inflammatory diseases that share similarities.

OBJECTIVE

To identify the histological and immunohistochemical (IHC) features of GA and sarcoidosis.

METHODS

A retrospective review of 36 patients with GA and 26 with sarcoidosis was performed. Results from hematoxylin and eosin (H&E) staining and IHC staining of MMP-9 and pSTAT1 within the skin lesions of GA and sarcoidosis were analyzed, and random forest was applied for developing a predictive model.

RESULTS

Significantly greater expressions of MMP-9 (especially in elastic fibers, EFs, < 0.0001) and pSTAT1 ( = 0.0003) were observed in lesion samples of GA versus sarcoidosis patients. In GA patients, MMP-9 was significantly upregulated in the interstitial type ( = 0.0222), while staining of pSTAT1 was positively correlated with the area of mucinous collagen in palisading GA ( = 0.5356, = 0.0484). In sarcoidosis patients, MMP-9 ( = -0.7127, = 0.0009) and pSTAT1 ( = -0.5604, = 0.0067) were found to show stronger expressions in lesions with less lymphocyte infiltration. The predictive model demonstrated an AUC of 0.9675.

CONCLUSION

These results indicate that MMP-9 and pSTAT1 might exert roles in granulomatous inflammation in different modes, and the presence of more robust MMP-9 staining in EFs appears to be more suggestive of GA.

摘要

背景

环状肉芽肿（GA）和结节病是具有相似特征的肉芽肿性炎症性疾病。

目的

确定 GA 和结节病的组织学和免疫组织化学（IHC）特征。

方法

对 36 例 GA 患者和 26 例结节病患者进行回顾性研究。分析 GA 和结节病皮损组织中 MMP-9 和 pSTAT1 的苏木精和伊红（H&E）染色和 IHC 染色结果，并应用随机森林开发预测模型。

结果

GA 患者病变组织中 MMP-9（尤其是弹性纤维， < 0.0001）和 pSTAT1（ = 0.0003）的表达明显高于结节病患者。在 GA 患者中，MMP-9 在间质型中显著上调（ = 0.0222），而 pSTAT1 的染色与栅栏状 GA 中粘蛋白胶原的面积呈正相关（ = 0.5356， = 0.0484）。在结节病患者中，MMP-9（ = -0.7127， = 0.0009）和 pSTAT1（ = -0.5604， = 0.0067）在淋巴细胞浸润较少的病变中表达更强。预测模型的 AUC 为 0.9675。