Su Ling, Zhang Man, Zhang Yudie, Chen Yingchun, Yang Liying, Wang Yongmei, Song Yangbo, Gong Lei
Shandong Academy of Grape, Shandong Academy of Agricultural Sciences, Jinan, China.
College of Horticulture Science and Technology, Hebei Normal University of Science and Technology, Hebei, China.
Front Plant Sci. 2023 Mar 7;14:1119749. doi: 10.3389/fpls.2023.1119749. eCollection 2023.
The formation of color in plants is significantly dependent on anthocyaninpigments. Grape species vary in color due to the differences in anthocyanin accumulation. It is widely recognized that both biotic and abiotic conditions may have an impact on anthocyanin synthesis in plants. The underlying molecular mechanisms by which external application of hyperoside impacts anthocyanin formation in grapes, however, have received little attention.
In the current study,the transcriptome of Gemstone seedless grape was examined using high-throughput RNA sequencing at various developmental stages reply to both control and hyperoside treatments.
The results of this study suggested that the major genes controlling anthocyanin accumulation in response to the externalinjection of hyperoside could be VvMYB62, VvPAL, VvCHS, and VvF3'5'H.Quantitative reverse transcription PCR (RT-qPCR) results were used to confirm the changes in the expression levels of the genes encoding the anthocyanin biosynthesis pathway under the control and hyperoside treatments. Using a transient transformation system, it was discovered that VvMYB62 was shown to regulate the anthocyanin accumulation at both the transcriptional and posttranslational levels and could be influenced by the external administration of hyperoside. In grape embryogenic calli, hyperoside could specifically suppress theexpression of VvMYB62 and anthocyanin accumulation. In this instance, the VvMYB62 characterisation brought attention to the significance of exogenous hyperoside-induced anthocyanin accumulation. Therefore, the results demonstrated that VvMYB62 could be hindered in the process of grape during anthocyanin accumulation caused by hyperoside.
These findings offer excellent candidate genes in the future breeding of novel grape varieties in addition to serving as a crucial reference for understanding the underlying molecular processes of hyperoside suppression of anthocyanin formation in plants.
植物颜色的形成很大程度上依赖于花青素色素。由于花青素积累的差异，葡萄品种在颜色上存在差异。人们普遍认识到，生物和非生物条件都可能对植物中的花青素合成产生影响。然而，表儿茶素外部施用影响葡萄中花青素形成的潜在分子机制却很少受到关注。
在本研究中，使用高通量RNA测序技术检测了宝石无核葡萄在不同发育阶段对对照和表儿茶素处理的转录组。
本研究结果表明，响应表儿茶素外部注射控制花青素积累的主要基因可能是VvMYB62、VvPAL、VvCHS和VvF3'5'H。定量逆转录PCR（RT-qPCR）结果用于确认对照和表儿茶素处理下花青素生物合成途径编码基因表达水平的变化。使用瞬时转化系统，发现VvMYB62在转录和翻译后水平上均调节花青素积累，并且可能受到表儿茶素外部施用的影响。在葡萄胚性愈伤组织中，表儿茶素可以特异性抑制VvMYB62的表达和花青素积累。在这种情况下，VvMYB62的特征引起了人们对外源表儿茶素诱导花青素积累重要性的关注。因此，结果表明在表儿茶素引起的葡萄花青素积累过程中，VvMYB62可能会受到阻碍。
这些发现除了为理解表儿茶素抑制植物中花青素形成的潜在分子过程提供关键参考外，还为未来新型葡萄品种的育种提供了优秀的候选基因。
