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查尔酮异构酶——[具体植物名称]中花青素生物合成的关键酶

Chalcone Isomerase a Key Enzyme for Anthocyanin Biosynthesis in .

作者信息

Sun Wei, Shen Huan, Xu Hui, Tang Xiaoxin, Tang Ming, Ju Zhigang, Yi Yin

机构信息

Key Laboratory of State Forestry Administration on Biodiversity Conservation in Karst Mountainous Areas of Southwestern China, School of Life Sciences, Guizhou Normal University, Guiyang, China.

Key Laboratory of Plant Physiology and Development Regulation, School of Life Sciences, Guizhou Normal University, Guiyang, China.

出版信息

Front Plant Sci. 2019 Jul 9;10:865. doi: 10.3389/fpls.2019.00865. eCollection 2019.

DOI:10.3389/fpls.2019.00865
PMID:31338101
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6629912/
Abstract

Anthocyanins are distributed ubiquitously to terrestrial plants and chalcone isomerase (CHI) catalyzes the stereospecific isomerization of chalcones - a committed step in the anthocyanin biosynthesis pathway. In this study, one gene encoding CHI was isolated from and designated as . Multiple sequence alignments and phylogenetic analysis revealed that OjCHI had the conserved CHI active site residues and was classified into type I CHI group. In order to better understand the mechanisms of anthocyanin synthesis in , integrative analysis between metabolites and expression was conducted. The results showed expression matched the accumulation patterns of anthocyanins not only in different tissues but also during the flower developmental stages, suggesting the potential roles of in the biosynthesis of anthocyanin. Then biochemical analysis indicated that recombinant OjCHI protein exhibited a typical type I CHI activity which catalyzed the production of naringenin from naringenin chalcone. Moreover, expressing in Arabidopsis mutant restored the anthocyanins and flavonols phenotype of hypocotyl, cotyledon and seed coat, indicating its function as a chalcone isomerase . In summary, our findings reveal the as well as functions of OjCHI and provide a resource to understand the mechanism of anthocyanin biosynthesis in .

摘要

花青素广泛分布于陆生植物中，查尔酮异构酶（CHI）催化查尔酮的立体特异性异构化，这是花青素生物合成途径中的关键步骤。在本研究中，从[具体来源未给出]分离出一个编码CHI的基因，并将其命名为[具体名称未给出]。多序列比对和系统发育分析表明，OjCHI具有保守的CHI活性位点残基，属于I型CHI组。为了更好地了解[具体植物名称未给出]中花青素合成的机制，对代谢物和[具体基因名称未给出]表达进行了综合分析。结果表明，[具体基因名称未给出]表达不仅与不同组织中花青素的积累模式相匹配，而且与花发育阶段的积累模式相匹配，表明[具体基因名称未给出]在花青素生物合成中具有潜在作用。然后生化分析表明，重组OjCHI蛋白表现出典型的I型CHI活性，催化柚皮素查尔酮生成柚皮素。此外，在拟南芥[具体突变体名称未给出]中表达[具体基因名称未给出]恢复了下胚轴、子叶和种皮的花青素和黄酮醇表型，表明其作为查尔酮异构酶的功能。总之，我们的研究结果揭示了OjCHI的[具体功能未详细说明]以及[具体功能未详细说明]功能，并为理解[具体植物名称未给出]中花青素生物合成机制提供了资源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c320/6629912/caf50033b98b/fpls-10-00865-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c320/6629912/086678d21ffd/fpls-10-00865-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c320/6629912/3cc9e669baa4/fpls-10-00865-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c320/6629912/612d71d8e864/fpls-10-00865-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c320/6629912/60ea666bc53c/fpls-10-00865-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c320/6629912/f545c3128c9d/fpls-10-00865-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c320/6629912/58c72105cd5c/fpls-10-00865-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c320/6629912/d3bc90c85a38/fpls-10-00865-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c320/6629912/caf50033b98b/fpls-10-00865-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c320/6629912/086678d21ffd/fpls-10-00865-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c320/6629912/3cc9e669baa4/fpls-10-00865-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c320/6629912/612d71d8e864/fpls-10-00865-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c320/6629912/60ea666bc53c/fpls-10-00865-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c320/6629912/f545c3128c9d/fpls-10-00865-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c320/6629912/58c72105cd5c/fpls-10-00865-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c320/6629912/d3bc90c85a38/fpls-10-00865-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c320/6629912/caf50033b98b/fpls-10-00865-g008.jpg

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