Sarwar G, Matayoshi S, Oda H
Department of Bacteriology, Faculty of Medicine, Kagoshima University.
Microbiol Immunol. 1987;31(9):869-77. doi: 10.1111/j.1348-0421.1987.tb03148.x.
A mixture of extracellular carrageenases was isolated from the cell-free medium of a culture of marine Cytophaga sp. 1k-C783 grown on ZoBell 2216 E broth with 0.1% commercial carrageenan. A single active peak of kappa-carrageenase was separated and purified from the mixture by ammonium sulfate precipitation, ion-exchange chromatography, and Sephadex G-200 gel filtration chromatography. Molecular weight of the purified kappa-carrageenase was estimated as 100,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The purified kappa-carrageenase had pH optimum 7.6 and temperature optimum 25 C.
从在含有0.1%商业角叉菜胶的佐贝尔2216E肉汤中培养的海洋纤维粘细菌1k-C783的无细胞培养基中分离出一种细胞外角叉菜胶酶混合物。通过硫酸铵沉淀、离子交换色谱和葡聚糖G-200凝胶过滤色谱从该混合物中分离并纯化出一个κ-角叉菜胶酶活性峰。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）估计纯化后的κ-角叉菜胶酶分子量为100,000。纯化后的κ-角叉菜胶酶最适pH为7.6，最适温度为25℃。
