Lockshin A
Cancer Research Laboratory, St. Joseph Hospital, Houston, TX 77002.
Neoplasma. 1987;34(5):545-55.
A method for detecting the uptake of 125I-labeled monoclonal antibody by human tumors xenografted into nude mice is described. After implantation of the tumor intramuscularly in one of the forelimbs, radioactivity was measured in the area of tumor inoculation and in the contralateral normal forelimb. For these measurements, a lead apparatus was constructed which shielded over 99% of the whole body radioactivity and permitted efficient counting of 125I in the exposed portion of each forelimb. For each tumor an uptake ratio (UR) was calculated for the radioactivity in the tumor compared to muscle. With this shield apparatus, reproducible, sensitive and relatively rapid determinations of UR values can be obtained. Antibody localization can be detected in some tumors which are nonpalpable or very small and for tumors of up to 2 cm3 in volume. Whole-body radioactivity ranging from less than 0.037 MBq to higher levels suitable for radioimmunoscintigraphy could be utilized in this procedure.
本文描述了一种检测接种于裸鼠的人肿瘤对¹²⁵I标记单克隆抗体摄取情况的方法。将肿瘤肌肉内植入一侧前肢后，在肿瘤接种部位及对侧正常前肢测量放射性。为进行这些测量，构建了一种铅制装置，该装置能屏蔽全身超过99%的放射性，并能对每个前肢暴露部分的¹²⁵I进行有效计数。对于每个肿瘤，计算肿瘤放射性与肌肉放射性的摄取比（UR）。使用该屏蔽装置，可获得可重复、灵敏且相对快速的UR值测定结果。在一些无法触及或非常小的肿瘤以及体积达2 cm³的肿瘤中均可检测到抗体定位。此方法可利用全身放射性范围从小于0.037 MBq到适用于放射免疫闪烁显像的更高水平的放射性。
