Anand Bibin G, Shejale Kiran P, Rajesh Kumar R, Thangam Ramar, Prajapati Kailash Prasad, Kar Karunakar, Mala R
Biomolecular Self Assembly Lab, Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu-603203, India; Biophysical and Biomaterials Research Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi- 110067, India.
Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 37673, Republic of Korea.
Biomater Adv. 2023 May;148:213346. doi: 10.1016/j.bioadv.2023.213346. Epub 2023 Feb 16.
Controlling the growth of biofilm on orthodontic material has become a difficult challenge in modern dentistry. The antibacterial efficacy of currently used orthodontic material becomes limited due to the higher affinity of oral microbial flora for plaque formation on the material surface. Thus it is crutial to device an efficient strategy to prevent plaque buildup caused by pathogenic microbiota. In this work, we have fabricated a bioactive orthodontic wire using titanium nanoparticles (TiONPs) and silver nanoparticles (AgNPs). AgNPs were synthesized from the extracts of Ocimum sanctum, Ocimum tenuiflorum, Solanum surattense, and Syzygium aromaticum, while the TiONPs were synthesized by the Sol-Gel method. The nanoparticles were characterized by various biophysical techniques. The surface of the dental wire was molded by functionalizing these AgNPs followed by an additional coating of TiONPs. Functionalized dental wires were found to counteract the formation of tenacious intraoral biofilm, and showed an enhanced anti-bacterial effect against Multi-Drug Resistant (MDR) bacteria isolated from patients with various dental ailments. Data revealed that such surface coating counteracts the bacterial pathogens by inducing the leakage of Ag ions which eventually disrupts the cell membrane as confirmed from TEM micrographs. The results offer a significant opportunity for innovations in developing nanoparticle-based formulations to modify or fabricate an effective orthodontic material.
控制正畸材料上生物膜的生长已成为现代牙科领域一项艰巨的挑战。由于口腔微生物菌群对材料表面菌斑形成具有更高的亲和力，目前使用的正畸材料的抗菌效果变得有限。因此，设计一种有效的策略来预防由致病微生物群引起的菌斑堆积至关重要。在这项工作中，我们使用钛纳米颗粒（TiONPs）和银纳米颗粒（AgNPs）制备了一种生物活性正畸丝。AgNPs是从神圣罗勒、细叶罗勒、刺天茄和丁香的提取物中合成的，而TiONPs是通过溶胶-凝胶法合成的。通过各种生物物理技术对纳米颗粒进行了表征。通过对这些AgNPs进行功能化处理，然后再额外涂覆TiONPs，对正畸丝表面进行了成型。发现功能化的正畸丝能够对抗口腔内坚韧生物膜的形成，并对从患有各种牙病的患者中分离出的多重耐药（MDR）细菌表现出增强的抗菌效果。数据显示，这种表面涂层通过诱导银离子泄漏来对抗细菌病原体，最终破坏细胞膜，这一点从透射电子显微镜图像中得到了证实。这些结果为开发基于纳米颗粒的配方以改性或制造有效的正畸材料提供了重大的创新机遇。
