Department of Agricultural Biotechnology, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Republic of Korea.
Department of Molecular Medicine, College of Medicine, Ewha Womans University, Seoul, 07804, Republic of Korea.
Tissue Eng Regen Med. 2023 Apr;20(2):155-156. doi: 10.1007/s13770-023-00537-w. Epub 2023 Mar 25.
Bone morphogenic protein-2 (BMP-2)-conjugated three-dimensional (3-D)-printed poly (L-lactic acid)(PLLA) scaffold is likely promising as an effective bone substitute for enhancing bone regeneration of massive bone defects caused by tumor resection, traumatic injury, or congenital diseases. The authors developed a new bone substitute using a novel strategy composed of 3-D-printed PLLA scaffolds through a sequential coating of catechol-conjugated alginate (C-AL), BMP-2, and collagen (CO). The 3-D-printed PLLA scaffold was successfully obtained with 5 mm of diameter, 1 mm of thickness, 400 μm of pore size, 187-230 μm of grid thickness, and 82% of porosity. Alkaline phosphatase (ALP) activity of the BMP-2-immobilized PLLA scaffold in MC3T3-E1 and W-20-17 cells was more increased than BMP-2 itself due to the controlled release of BMP-2 from the scaffold. Tenfold new bone formation for the BMP-2-immobilized PLLA scaffold was obtained by micro-CT analysis than PLLA scaffold without BMP-2 weeks after 4 weeks of transplantation model mouse. Further another big animal model study should be performed before clinical trials.
骨形态发生蛋白 2（BMP-2）偶联的三维（3-D）打印聚（L-乳酸）（PLLA）支架有望成为一种有效的骨替代物，可增强因肿瘤切除、创伤或先天性疾病引起的大骨缺损的骨再生。作者开发了一种新的骨替代物，该替代物采用了一种新策略，通过依次涂覆儿茶酚偶联藻酸盐（C-AL）、BMP-2 和胶原蛋白（CO）来制造 3-D 打印 PLLA 支架。成功获得了直径为 5mm、厚度为 1mm、孔径为 400μm、网格厚度为 187-230μm、孔隙率为 82%的 3-D 打印 PLLA 支架。由于 BMP-2 从支架中的受控释放，固定在 PLLA 支架上的碱性磷酸酶（ALP）活性比 BMP-2 本身更高，BMP-2 固定在 PLLA 支架上的 MC3T3-E1 和 W-20-17 细胞中的 ALP 活性更高。在移植模型小鼠 4 周后，BMP-2 固定 PLLA 支架的微 CT 分析显示新骨形成增加了 10 倍，而没有 BMP-2 的 PLLA 支架则没有。在临床试验之前，应该进行进一步的大动物模型研究。
