生理盐水负荷大鼠体内调节催产素分泌的内源性阿片机制活性的时间变化。

Temporal changes in the activity of endogenous opioid mechanisms regulating oxytocin secretion in saline-loaded rats.

作者信息

Carter D A, Lightman S L

机构信息

Medical Unit, Charing Cross and Westminster Medical School, Westminster Hospital, London U.K.

出版信息

Neurosci Lett. 1987 Nov 23;82(2):191-5. doi: 10.1016/0304-3940(87)90128-5.

DOI:10.1016/0304-3940(87)90128-5

PMID:3696493

Abstract

The effect of the opiate antagonist naloxone on neurohypophyseal hormone secretion has been investigated in rats given 2% NaCl to drink for 0-9 days. Naloxone elicited a rise in plasma levels of oxytocin (OT), but not arginine-vasopressin (AVP), on days 1-3 only, the greatest response being on day 2. In a further experiment, the OT response to an acute stimulus (1.5 M NaCl, i.p.) was found to be similar in controls and rats drinking 2% NaCl for 7 days, indicating that depletion of readily releasable hormone was not the cause of the loss of sensitivity to naloxone; rather a down-regulation of opiate receptor mechanisms may be responsible. The temporal changes in opiate antagonist-sensitivity in saline-loaded rats may provide a functional paradigm for the investigation of regulatory opiate mechanisms in neural tissue.

摘要

在给大鼠饮用2%氯化钠溶液0至9天的情况下，研究了阿片类拮抗剂纳洛酮对神经垂体激素分泌的影响。仅在第1至3天，纳洛酮使血浆催产素（OT）水平升高，但不影响精氨酸加压素（AVP）水平，最大反应出现在第2天。在进一步的实验中，发现对照组和饮用2%氯化钠溶液7天的大鼠对急性刺激（腹腔注射1.5 M氯化钠）的OT反应相似，这表明易于释放的激素耗竭不是对纳洛酮敏感性丧失的原因；相反，阿片受体机制的下调可能是原因所在。盐负荷大鼠中阿片拮抗剂敏感性的时间变化可能为研究神经组织中调节性阿片机制提供一个功能范例。