State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, People's Republic of China.
Leicester International Institute, Dalian University of Technology, Panjin 124221, People's Republic of China.
Food Chem. 2023 Aug 30;418:135891. doi: 10.1016/j.foodchem.2023.135891. Epub 2023 Mar 23.
Herein, we have introduced hybridization chain reaction (HCR) into the photonic crystals (PhCs) hydrogel, for the first time, realizing HCR for inducing the change of the optical signal of PhCs hydrogel and using this hydrogel as a sensor for determination of the aflatoxin B1 (AFB1). By using specific sequences as the cross-linker, the extension of the cross-linker by HCR drives the swelling of the hydrogel, and the optical property of 2D PhCs array converts this swelling into a change of the Debye diffraction ring. Moreover, by further selecting the aptamer to construct the cross-linker, the hydrogel is also endowed with a unique capability for AFB1, making the hydrogel a novel sensor based on the signal amplification strategy. The results show that the designed hairpin DNAs can effectively trigger the HCR and cause the swelling of hydrogel, and the hydrogel sensor has a good determination performance and high specific recognition for AFB1.
在这里，我们首次将杂交链式反应（HCR）引入光子晶体（PhC）水凝胶中，实现了 HCR 诱导 PhC 水凝胶光学信号变化，并将该水凝胶用作测定黄曲霉毒素 B1（AFB1）的传感器。通过使用特定序列作为交联剂，HCR 驱动交联剂的延伸导致水凝胶溶胀，二维 PhC 阵列的光学性质将这种溶胀转化为德拜衍射环的变化。此外，通过进一步选择适体来构建交联剂，水凝胶还赋予了其对 AFB1 的独特识别能力，使水凝胶成为一种基于信号放大策略的新型传感器。结果表明，设计的发夹 DNA 可以有效地引发 HCR 并引起水凝胶溶胀，并且水凝胶传感器对 AFB1 具有良好的测定性能和高特异性识别。
