College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, 271018, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai'an, 271018, China.
College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, 271018, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai'an, 271018, China.
Poult Sci. 2023 May;102(5):102591. doi: 10.1016/j.psj.2023.102591. Epub 2023 Feb 16.
Epidemiologic investigations in recent years have shown that the detection rate of avian hepatitis E virus (HEV) in chicken flocks is increasing in China. Nevertheless, effective prevention and control measures are still lacking. In this study, specific pathogen-free (SPF) chicken serum against HEV was prepared using recombinant HEV open reading frames (ORF2 and ORF3) proteins as immunogens. An SPF chicken infection model was established by intravenous inoculation of chick embryos. Swab samples were collected at 7, 14, 21, and 28 d of age and used to detect avian HEV load, along with other indicators, by fluorescence quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) assay. The therapeutic effects on blocking vertical HEV transmission were observed, by using the methods of antibody application alone, mixed, or combined application of each of the 2 antibodies with type I interferon. The results showed that type I interferon alone or in combination with antiserum reduced the positive rate of HEV from 100 to 62.5% and 25%, respectively. However, the avian HEV-positivity rate was reduced to 75, 50, and 37.5% after type I interferon was used alone or in combination with antisera against ORF2 and ORF3, respectively. The inhibitory effect of type I interferon alone or in combination with an antiserum, on HEV replication was more significant in cells than in vivo. In this study, the inhibitory effect of type I interferon alone or in combination with an antiserum on avian HEV replication was observed in vitro and in vivo, providing the necessary technical reserve for disease prevention and control.
近年来的流行病学调查表明，我国鸡群中禽戊型肝炎病毒（HEV）的检出率呈上升趋势，但目前仍缺乏有效的防控措施。本研究以重组 HEV 开放阅读框（ORF2 和 ORF3）蛋白为免疫原，制备抗禽 HEV 的特异性无病原体（SPF）鸡血清。通过静脉接种鸡胚建立 SPF 鸡感染模型。分别于 7、14、21 和 28 日龄采集拭子样本，采用荧光定量 RT-PCR 法检测禽 HEV 载量及其他指标。观察了单独、混合或联合应用两种抗体及 I 型干扰素对阻断垂直传播禽 HEV 的治疗效果。结果显示，I 型干扰素单独或联合抗血清可将 HEV 的阳性率从 100%降低至 62.5%和 25%，但 I 型干扰素单独或联合抗 ORF2 和 ORF3 血清可将禽 HEV 的阳性率分别降低至 75%、50%和 37.5%。I 型干扰素单独或联合抗血清对 HEV 复制的抑制作用在细胞水平上强于体内。本研究在体内外观察到 I 型干扰素单独或联合抗血清对禽 HEV 复制的抑制作用，为疾病的防控提供了必要的技术储备。
