Torrella F, Guerrero R, Seidler R J
Can J Microbiol. 1978 Nov;24(11):1387-94. doi: 10.1139/m78-222.
Cultures of Bdellovibrio isolated from different geographic locations have been studied in terms of deoxyribonucleic acid analysis (% G + C, genome size, and DNA hybridization), cytochrome spectrum, and host range. Isolates of the genus exhibit a broad range of % G + C ranging from 37 to 51% and the genome sizes extend from 1300 x 10(6) to 1700 x 10(6) daltons. DNA hybridization continues to reveal a high level of genetic heterogeneity. Bdellovibrio 3294 exhibits 32% relative reassociation to Bdellovibrio W, 37% to Bdellovibrio stolpii Uki2, and an undetectible level to Bdellovibrio starrii A3.12 Bdellovibrio W is 23% related to B. starri A3.12 and 28.5% to B. stolpii Uki2. For the first time differential absorption techniques have revealed peaks of cytochrome b. The analysis of the cytochrome spectrum seems to be limited as a taxonomic tool since most of the recent isolates studied share a common cytochrome spectrum. Host-range studies have been found to be dependent on the experimental conditions, and with the exception of one isolate (B. starrii A3.12) the taxonomic significance of such techniques must be taken with caution.
已从脱氧核糖核酸分析（%G + C、基因组大小和DNA杂交）、细胞色素光谱和宿主范围等方面对从不同地理位置分离出的蛭弧菌培养物进行了研究。该属的分离株表现出广泛的%G + C范围，从37%到51%不等，基因组大小从1300×10⁶到1700×10⁶道尔顿。DNA杂交继续显示出高水平的遗传异质性。蛭弧菌3294与蛭弧菌W的相对重结合率为32%，与蛭弧菌斯托尔皮氏菌Uki2的相对重结合率为37%，与星状蛭弧菌A3.12的相对重结合率无法检测到。蛭弧菌W与星状蛭弧菌A3.12的相关性为23%，与蛭弧菌斯托尔皮氏菌Uki2的相关性为28.5%。差分吸收技术首次揭示了细胞色素b的峰。细胞色素光谱分析作为一种分类工具似乎受到限制，因为最近研究的大多数分离株都具有共同的细胞色素光谱。已发现宿主范围研究取决于实验条件，除了一个分离株（星状蛭弧菌A3.12）外，此类技术的分类学意义必须谨慎对待。
