Zhao Zhengzheng, Yu Chan, Yang Chongrui, Gao Bingqi, Jiménez Núria, Wang Chen, Li Fang, Ao Yue, Zheng Longyu, Huang Feng, Tomberlin Jeffery K, Ren Zhuqing, Yu Ziniu, Zhang Jibin, Cai Minmin
State Key Laboratory of Agricultural Microbiology, National Engineering Research Center of Microbial Pesticides, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Hubei Hongshan Laboratory, Wuhan 430070, Hubei, China.
State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, China.
Sci Total Environ. 2023 Jun 25;879:163065. doi: 10.1016/j.scitotenv.2023.163065. Epub 2023 Mar 24.
The increasing prevalence of antibiotic resistance genes (ARGs) in animal manure has attracted considerable attention because of their potential contribution to the development of multidrug resistance worldwide. Insect technology may be a promising alternative for the rapid attenuation of ARGs in manure; however, the underlying mechanism remains unclear. This study aimed to evaluate the effects of black soldier fly (BSF, Hermetia illucens [L.]) larvae conversion combined with composting on ARGs dynamics in swine manure and to uncover the mechanisms through metagenomic analysis. Compared to natural composting (i.e. without BSF), BSFL conversion combined with composting reduced the absolute abundance of ARGs by 93.2 % within 28 days. The rapid degradation of antibiotics and nutrient reformulation during BSFL conversion combined with composting indirectly altered manure bacterial communities, resulting in a lower abundance and richness of ARGs. The number of main antibiotic-resistant bacteria (e.g., Prevotella, Ruminococcus) decreased by 74.9 %, while their potential antagonistic bacteria (e.g., Bacillus, Pseudomonas) increased by 128.7 %. The number of antibiotic-resistant pathogenic bacteria (e.g., Selenomonas, Paenalcaligenes) decreased by 88.3 %, and the average number of ARGs carried by each human pathogenic bacterial genus declined by 55.8 %. BSF larvae gut microbiota (e.g., Clostridium butyricum, C. bornimense) could help reduce the risk of multidrug-resistant pathogens. These results provide insight into a novel approach to mitigate multidrug resistance from the animal industry in the environment by using insect technology combined with composting, in particular in light of the global "One Health" requirements.
动物粪便中抗生素抗性基因（ARGs）的日益普遍因其对全球多重耐药性发展的潜在影响而备受关注。昆虫技术可能是快速降低粪便中ARGs的一种有前景的替代方法；然而，其潜在机制仍不清楚。本研究旨在评估黑水虻（BSF，亮斑扁角水虻[L.]）幼虫转化结合堆肥对猪粪中ARGs动态的影响，并通过宏基因组分析揭示其机制。与自然堆肥（即不使用黑水虻）相比，黑水虻幼虫转化结合堆肥在28天内使ARGs的绝对丰度降低了93.2%。黑水虻幼虫转化结合堆肥过程中抗生素的快速降解和养分重新配方间接改变了粪便细菌群落，导致ARGs的丰度和丰富度降低。主要抗生素抗性细菌（如普雷沃氏菌、瘤胃球菌）的数量减少了74.9%，而它们的潜在拮抗细菌（如芽孢杆菌、假单胞菌）增加了128.7%。抗生素抗性病原菌（如栖瘤胃普雷沃氏菌、产碱假单胞菌）的数量减少了88.3%，每个人类致病细菌属携带的ARGs平均数量下降了55.8%。黑水虻幼虫肠道微生物群（如丁酸梭菌、博尔尼梭菌）有助于降低多重耐药病原体的风险。这些结果为通过结合昆虫技术和堆肥来减轻环境中动物养殖业多重耐药性的新方法提供了见解，特别是鉴于全球“同一健康”的要求。
