State Key Laboratory of Agricultural Microbiology, National Engineering Research Center of Microbial Pesticides, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China; University of Messina, Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, Messina, Italy.
State Key Laboratory of Agricultural Microbiology, National Engineering Research Center of Microbial Pesticides, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
Waste Manag. 2020 Feb 1;102:312-318. doi: 10.1016/j.wasman.2019.10.055. Epub 2019 Nov 25.
Black soldier fly (BSF) is used for the management of organic waste, but research has hardly explored the effect of companion bacteria when chicken manure (CHM) is converted to insect biomass. In this study, we isolated nine bacterial species (FE01, FE02, FE03, FE04, FE05, FE06, FE07, FE08, FE09) from BSF eggs and one (BSF-CL) from the larval gut. These companion bacteria were inoculated into CHM along with BSF larvae (BSFL). Larval growth and manure conversion rates were determined. Results indicated that almost all bacteria individual bacteria in this study significantly promote BSFL growth. BSFL reared in manure with the species Kocuria marina (FE01), Lysinibacillus boronitolerans (FE04), Proteus mirabilis (FE08) and Bacillus subtilis (BSF-CL) had higher weight gain and manure reduction rates compared to the control. These four strains used were then examined as a poly-bacteria community experiment to determine BSFL growth and manure conversion. Manure inoculated with the poly-bacteria Group3 (FE01:FE04:FE08:BSF-CL = 4:1:1:1) and then fed to BSFL resulted in 28.6% more weight gain than the control. The greatest manure reduction rate (52.91%) was reached when companion bacteria were mixed at a ratio of 1:1:1:4. Additionally, the companion bacteria influenced the nutritional value of BSFL. Crude protein content in Group1 (FE01:FE04:FE08:BSF-CL = 1:1:1:1) was significantly larger than that of the control. Crude fat content in Group3 was significantly larger than that of the control. BSFL companion bacteria and their poly-bacteria compound improved manure conversion efficiency and nutrient accumulation in BSFL, reduced CHM quantity, increased larvae biomass, with potential economic gains in CHM management.
黑水虻（BSF）被用于有机废物管理，但在将鸡粪（CHM）转化为昆虫生物量时，研究几乎没有探索共生细菌的作用。在这项研究中，我们从 BSF 卵中分离出了 9 种细菌（FE01、FE02、FE03、FE04、FE05、FE06、FE07、FE08、FE09）和一种（BSF-CL）从幼虫肠道中分离出来。这些共生细菌与 BSF 幼虫（BSFL）一起接种到 CHM 中。测定了幼虫生长和粪便转化率。结果表明，本研究中的几乎所有细菌个体都显著促进了 BSFL 的生长。与对照相比，在含有海洋分枝杆菌（FE01）、硼耐受解淀粉芽孢杆菌（FE04）、奇异变形杆菌（FE08）和枯草芽孢杆菌（BSF-CL）的粪便中饲养的 BSFL 体重增加和粪便减少率更高。然后，将这四种菌株作为多细菌群落实验进行检查，以确定 BSFL 的生长和粪便转化率。用多细菌组 3（FE01：FE04：FE08：BSF-CL=4：1：1：1）接种的粪便并喂食 BSFL 比对照体重增加了 28.6%。当共生细菌以 1:1:1:4 的比例混合时，达到了最高的粪便减少率（52.91%）。此外，共生细菌影响了 BSFL 的营养价值。组 1（FE01：FE04：FE08：BSF-CL=1：1：1：1）中的粗蛋白含量显著大于对照。组 3 中的粗脂肪含量显著大于对照。BSFL 共生细菌及其多细菌化合物提高了 CHM 管理中粪便转化率效率和 BSFL 中的营养物质积累，减少了 CHM 量，增加了幼虫生物量，具有潜在的经济效益。
