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基于脂催化褐煤厌氧发酵转化生物甲烷的宏基因组学特征分析。

Metagenomic characterization of biomethane transformation by lipid-catalyzed anaerobic fermentation of lignite.

机构信息

Research Institute of Petroleum Exploration & Development, Beijing, 100083, China.

School of Energy Science and Engineering, Henan Polytechnic University, Jiaozuo, 454000, China.

出版信息

Environ Res. 2023 Jun 15;227:115777. doi: 10.1016/j.envres.2023.115777. Epub 2023 Mar 24.

DOI:10.1016/j.envres.2023.115777
PMID:36966989
Abstract

The present study aims at using lipid in a novel way to improve the efficiency of methane production from lignite anaerobic digestion. The obtained results showed an increase by 3.13 times of the cumulative biomethane content of lignite anaerobic fermentation, when 1.8 g lipid was added. The gene expression of functional metabolic enzymes was also found to be enhanced during the anaerobic fermentation. Moreover, the enzymes related to fatty acid degradation such as long-chain Acyl-CoA synthetase and Acyl-CoA dehydrogenase were increased by 1.72 and 10.48 times, respectively, which consequently, accelerated the conversion of fatty acid. Furthermore, the addition of lipid enhanced the carbon dioxide trophic and acetic acid trophic metabolic pathways. Hence, the addition of lipids was argued to promote the production of methane from lignite anaerobic fermentation, which provided a new insight for the conversion and utilization of lipid waste.

摘要

本研究旨在探索一种新方法,利用脂质来提高褐煤厌氧消化产甲烷的效率。结果表明,当添加 1.8 g 脂质时,褐煤厌氧发酵的累积生物甲烷含量增加了 3.13 倍。在厌氧发酵过程中,功能代谢酶的基因表达也得到了增强。此外,长链酰基辅酶 A 合成酶和酰基辅酶 A 脱氢酶等与脂肪酸降解相关的酶的活性分别增加了 1.72 倍和 10.48 倍,从而加速了脂肪酸的转化。此外,脂质的添加增强了二氧化碳营养和乙酸营养代谢途径。因此,添加脂质可以促进褐煤厌氧发酵产甲烷,为脂质废物的转化和利用提供了新的思路。

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