Li Ya, Chen Juan, Xie Min, Cao Yihui, Zhou Yan, Zhang Ruixian
Department of Clinical Laboratory, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China.
Department of Rheumatology and Immunology, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China.
Autoimmunity. 2023 Dec;56(1):2193361. doi: 10.1080/08916934.2023.2193361.
Systemic lupus erythematosus (SLE) is a chronic autoimmune disease. At present, the mechanism of non-coding RNA in renal injury in SLE patients is still unclear. A total of 64 DEcircRNAs, 75 DEmiRNAs, and 249 DEmRNAs were identified. We integrated 10 circRNAs, 10 miRNAs, and 88 target mRNAs into a circRNA-miRNA-mRNA network and obtained 9 hub genes (circ-0000006, miR-766-3p, miR-409-3p, miR-339-3p, miR-331-3p, miR-140-3p, miR-186-5p, miR-149-5p, PSME3). The ROC curve results showed that the diagnostic efficiency of 6 hub miRNA was higher than that of has_circ_0000006 and PSEME3. SsGSEA analysis revealed immune cell composition in SLE and control renal tissues, including 3 types of immune cells up-regulated (gamma delta T cell, effector memory CD4 T cell, central memory CD8 T cell) and 4 types down-regulated (memory B cell, mast cell, macrophage, immature dendritic cell, eosinophil) in SLE patients. In addition, PSME3 was negatively correlated with 3 up-regulated immune cells and positively correlated with 4 down-regulated immune cells in SLE patients. Our study provides a deeper understanding of the circRNA-related competing endogenous RNA regulatory mechanism in the renal injury of systemic lupus erythematosus.
系统性红斑狼疮（SLE）是一种慢性自身免疫性疾病。目前，SLE患者肾损伤中非编码RNA的机制仍不清楚。共鉴定出64种差异表达的环状RNA（DEcircRNAs）、75种差异表达的微小RNA（DEmiRNAs）和249种差异表达的信使RNA（DEmRNAs）。我们将10种环状RNA、10种微小RNA和88种靶信使RNA整合到一个环状RNA-微小RNA-信使RNA网络中，获得了9个枢纽基因（circ-0000006、miR-766-3p、miR-409-3p、miR-339-3p、miR-331-3p、miR-140-3p、miR-186-5p、miR-149-5p、PSME3）。ROC曲线结果显示，6种枢纽微小RNA的诊断效率高于has_circ_0000006和PSEME3。单样本基因集富集分析（SsGSEA）揭示了SLE和对照肾组织中的免疫细胞组成，包括SLE患者中上调的3种免疫细胞（γδT细胞、效应记忆CD4 T细胞、中枢记忆CD8 T细胞）和下调的4种免疫细胞（记忆B细胞、肥大细胞、巨噬细胞、未成熟树突状细胞、嗜酸性粒细胞）。此外，在SLE患者中，PSME3与3种上调的免疫细胞呈负相关，与4种下调的免疫细胞呈正相关。我们的研究为深入了解系统性红斑狼疮肾损伤中环状RNA相关的竞争性内源性RNA调控机制提供了依据。
