Hu Yajun, Zhang Junzhi, Cai Minglang, Chu Wuying, Hu Yi
Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agricultural University, Changsha Hunan 410128, China.
College of Animal Science and Technology, Hunan Agricultural University, Changsha Hunan 410128, China.
Aquac Nutr. 2023 Mar 16;2023:5533414. doi: 10.1155/2023/5533414. eCollection 2023.
An eight-week feeding trial explored the mechanism that supplemented methionine (0 g/kg, 4 g/kg, 8 g/kg, and 12 g/kg) in a high-fat diet (120 g/kg fat) on intestinal lipid transportation and gut microbiota of (initial weight 25.03 ± 0.13 g) based on the diet (60 g/kg fat), named as Con, HFD+M0, HFD+M4, HFD+M8, and HFD+M12, respectively. Compared with Con, gastric amylase, lipase, trypsin ( < 0.05), and intestinal lipase, amylase, trypsin, NaK -Adenosinetriphosphatase, depth of gastric fovea, and the number of intestinal villus goblet cells of HFD+M0 were markedly declined ( < 0.05), while intestinal high-density lipoprotein-cholesterol, very low-density lipoprotein-cholesterol and microsomal triglyceride transfer protein of HFD+M0 were markedly enhanced ( < 0.05); compared with HFD+M0, gastric lipase, amylase, trypsin, and intestinal lipase, trypsin, Na/K -Adenosinetriphosphatase, microsomal triglyceride transfer protein, very low-density lipoprotein-cholesterol, and apolipoprotein -A, the height of intestinal villus and the number of intestinal villus goblet cells of HFD+M8 were remarkably enhanced ( < 0.05). Compared with Con, intestinal , , , , of HFD + M0 were markedly down-regulated ( <0.05), while intestinal , , , , , , , , , , , , , , , , , , , , of HFD M0 were remarkably upregulated ( < 0.05); compared with HFD+M0, intestinal and of HFD+M8 were remarkably downregulated ( < 0.05), intestinal , , , , , , , , , , , , , , , , , , , , , , , , and were remarkably upregulated ( < 0.05). Compared with Con, the diversity of gut microbiota of HFD+M0 was significantly declined ( < 0.05), while the diversity of gut microbiota in HFD+M8 was significantly higher than that in HFD+M0 ( < 0.05). In conclusion, a high-fat methionine deficiency diet destroyed the intestinal barrier, reduced the capacity of intestinal digestion and absorption, and disrupted the balance of gut microbiota; supplemented methionine promoted the digestion and absorption of lipids, and also improved the balance of gut microbiota.
一项为期八周的饲养试验，探究了在高脂饮食（脂肪含量120 g/kg）中添加蛋氨酸（0 g/kg、4 g/kg、8 g/kg和12 g/kg）对初始体重为25.03±0.13 g的（实验对象未明确写出）基于60 g/kg脂肪的饮食（命名为Con）、HFD+M0、HFD+M4、HFD+M8和HFD+M12的肠道脂质转运和肠道微生物群的影响机制。与Con组相比，HFD+M0组的胃淀粉酶、脂肪酶、胰蛋白酶（P<0.05）以及肠脂肪酶、淀粉酶、胰蛋白酶、钠钾-三磷酸腺苷酶、胃小凹深度和肠绒毛杯状细胞数量均显著下降（P<0.05），而HFD+M0组的肠高密度脂蛋白胆固醇、极低密度脂蛋白胆固醇和微粒体甘油三酯转移蛋白则显著升高（P<0.05）；与HFD+M0组相比，HFD+M8组的胃脂肪酶、淀粉酶、胰蛋白酶以及肠脂肪酶、胰蛋白酶、钠/钾-三磷酸腺苷酶、微粒体甘油三酯转移蛋白、极低密度脂蛋白胆固醇和载脂蛋白-A、肠绒毛高度和肠绒毛杯状细胞数量均显著增加（P<0.05）。与Con组相比，HFD+M0组的肠道（此处原文不完整，推测为某些基因或蛋白名称）显著下调（P<0.05），而HFD+M0组的肠道（此处原文不完整，推测为某些基因或蛋白名称）显著上调（P<0.05）；与HFD+M0组相比，HFD+M8组的肠道（此处原文不完整，推测为某些基因或蛋白名称）显著下调（P<0.05），肠道（此处原文不完整，推测为某些基因或蛋白名称）显著上调（P<0.05）。与Con组相比，HFD+M0组肠道微生物群的多样性显著下降（P<0.05），而HFD+M8组肠道微生物群的多样性显著高于HFD+M0组（P<0.05）。总之，高脂蛋氨酸缺乏饮食破坏了肠道屏障，降低了肠道消化吸收能力，扰乱了肠道微生物群的平衡；补充蛋氨酸促进了脂质的消化吸收，也改善了肠道微生物群的平衡。
