Methionine-Mediated Regulation of Intestinal Lipid Transportation Induced by High-Fat Diet in Rice Field Eel ().

An eight-week feeding trial explored the mechanism that supplemented methionine (0 g/kg, 4 g/kg, 8 g/kg, and 12 g/kg) in a high-fat diet (120 g/kg fat) on intestinal lipid transportation and gut microbiota of (initial weight 25.03 ± 0.13 g) based on the diet (60 g/kg fat), named as Con, HFD+M0, HFD+M4, HFD+M8, and HFD+M12, respectively. Compared with Con, gastric amylase, lipase, trypsin ( < 0.05), and intestinal lipase, amylase, trypsin, NaK -Adenosinetriphosphatase, depth of gastric fovea, and the number of intestinal villus goblet cells of HFD+M0 were markedly declined ( < 0.05), while intestinal high-density lipoprotein-cholesterol, very low-density lipoprotein-cholesterol and microsomal triglyceride transfer protein of HFD+M0 were markedly enhanced ( < 0.05); compared with HFD+M0, gastric lipase, amylase, trypsin, and intestinal lipase, trypsin, Na/K -Adenosinetriphosphatase, microsomal triglyceride transfer protein, very low-density lipoprotein-cholesterol, and apolipoprotein -A, the height of intestinal villus and the number of intestinal villus goblet cells of HFD+M8 were remarkably enhanced ( < 0.05). Compared with Con, intestinal , , , , of HFD + M0 were markedly down-regulated ( <0.05), while intestinal , , , , , , , , , , , , , , , , , , , , of HFD M0 were remarkably upregulated ( < 0.05); compared with HFD+M0, intestinal and of HFD+M8 were remarkably downregulated ( < 0.05), intestinal , , , , , , , , , , , , , , , , , , , , , , , , and were remarkably upregulated ( < 0.05). Compared with Con, the diversity of gut microbiota of HFD+M0 was significantly declined ( < 0.05), while the diversity of gut microbiota in HFD+M8 was significantly higher than that in HFD+M0 ( < 0.05). In conclusion, a high-fat methionine deficiency diet destroyed the intestinal barrier, reduced the capacity of intestinal digestion and absorption, and disrupted the balance of gut microbiota; supplemented methionine promoted the digestion and absorption of lipids, and also improved the balance of gut microbiota.