Árpádffy-Lovas Tamás, Nagy Norbert
Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Szeged, Szeged, Hungary.
ELKH-SZTE Research Group of Cardiovascular Pharmacology, Eötvös Loránd Research Network, Szeged, Hungary.
Heliyon. 2023 Mar 13;9(3):e14440. doi: 10.1016/j.heliyon.2023.e14440. eCollection 2023 Mar.
The action potential forms the basis of cardiac pacemaking, conduction, and contraction. Action potentials can be recorded from numerous preparation types, including ventricular or atrial trabecules, Purkinje fibers, isolated cardiac myocytes. Numerous techniques are also available as well, such as the conventional microelectrode and the single-cell current clamp techniques, optical mapping, or modeling. With such a vast array of electrophysiological methods comes an array of available hardware and software solutions. In this work, we present a software with an intuitive graphical user interface, ActionPytential, that enables the analysis of any type of cardiac action potential, regardless of acquisition method or tissue type. In most available software tools, the analysis of continuous (gap-free) recordings often requires manual user interaction to segment the individual action potentials. We provide an automated solution for this, both for slow-response and for externally paced action potentials. As of now, ActionPytential calculates 34 parameters from each action potential. The most often utilized ones, including amplitude, maximal rate of depolarization, and action potential duration values, were validated on 1200 action potentials from human, dog, rabbit, guinea pig, and rat cardiac preparations. We also provide new parameters that were previously only measurable manually, including the position and the depth of the in potentials showing a spike-and-dome morphology. Further notable features include a Butterworth-type low-pass filter, the averaging of multiple potentials, automated corrections for baseline drifting, aided manual analysis, high-quality plots, and batch processing for any number of potentials. ActionPytential is available for all major platforms (Windows, MacOS, GNU + Linux, BSD).
动作电位构成了心脏起搏、传导和收缩的基础。动作电位可以从多种制备类型中记录，包括心室或心房小梁、浦肯野纤维、分离的心肌细胞。也有许多技术可用，如传统微电极和单细胞电流钳技术、光学映射或建模。有如此众多的电生理方法，就有一系列可用的硬件和软件解决方案。在这项工作中，我们展示了一款具有直观图形用户界面的软件ActionPytential，它能够分析任何类型的心脏动作电位，无论采集方法或组织类型如何。在大多数现有的软件工具中，对连续（无间隙）记录的分析通常需要用户手动交互来分割各个动作电位。我们为此提供了一种自动化解决方案，适用于慢反应动作电位和外部起搏动作电位。截至目前，ActionPytential从每个动作电位中计算34个参数。最常用的参数，包括幅度、最大去极化速率和动作电位持续时间值，在来自人类、狗、兔子、豚鼠和大鼠心脏制备的1200个动作电位上进行了验证。我们还提供了以前只能手动测量的新参数，包括显示尖峰 - 圆顶形态的电位的位置和深度。其他显著特点包括巴特沃斯型低通滤波器、多个电位的平均、基线漂移的自动校正、辅助手动分析、高质量绘图以及任意数量电位的批处理。ActionPytential适用于所有主要平台（Windows、MacOS、GNU + Linux、BSD）。
