大黄-桃仁这一植物药组合通过抑制Rho GTP酶改善子宫腺肌病。

Dahuang-Taoren, a botanical drug combination, ameliorates adenomyosis inhibiting Rho GTPases.

作者信息

Lei Ya, Fu Xianyun, Chen Minmin, Yi Yongli, Mao Ping, Peng Li, Qu Zhao

机构信息

The First College of Clinical Medical Science, China Three Gorges University, Yichang Central People's Hospital, Yichang, China.

Third-Grade Pharmacological Laboratory on Chinese Medicine Approved by State Administration of Traditional Chinese Medicine, Medical College, China Three Gorges University, Yichang, China.

出版信息

Front Pharmacol. 2023 Mar 6;14:1089004. doi: 10.3389/fphar.2023.1089004. eCollection 2023.

DOI:10.3389/fphar.2023.1089004

PMID:36969843

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10035534/

Abstract

Dahuang-Taoren (DT) is a classic combination of botanical drugs applied to treat pain-related diseases in ancient China. Today, DT is frequently applied for dysmenorrhea of adenomyosis (AM) in the clinic. Growing evidence indicates Rho GTPases may play an essential role in AM progression. However, the potential mechanism of DT on Rho GTPases in AM remains unclear. The expressions of Rho GTPases in the patients with AM were evaluated. Further, pituitary transplantation-induced AM mice and the primary AM endometrial stromal cells (AMESCs) were subjected to DT intervention. The results revealed that the expressions of Rho GTPases were significantly upregulated in both AM patients and AM mice. The DT could reduce pathological infiltration, relieve hyperalgesia, and alleviate cytoskeleton remodeling in AM mice. Besides, the migration and invasion of AMESCs were markedly inhibited after exposure to DT. These effects may be linked to the decreased Rho GTPases expression. The results may offer a novel explanation of DT against AM.

摘要

大黄-桃仁（DT）是中国古代用于治疗疼痛相关疾病的经典植物药组合。如今，DT在临床上常用于治疗子宫腺肌病（AM）的痛经。越来越多的证据表明，Rho GTPases可能在AM进展中起关键作用。然而，DT对AM中Rho GTPases的潜在作用机制仍不清楚。评估了AM患者中Rho GTPases的表达。此外，对垂体移植诱导的AM小鼠和原代AM子宫内膜基质细胞（AMESCs）进行了DT干预。结果显示，AM患者和AM小鼠中Rho GTPases的表达均显著上调。DT可减少AM小鼠的病理浸润，减轻痛觉过敏，并缓解细胞骨架重塑。此外，暴露于DT后，AMESCs的迁移和侵袭明显受到抑制。这些作用可能与Rho GTPases表达降低有关。该结果可能为DT治疗AM提供新的解释。