遮荫诱导的长非编码 RNA PUAR 通过抑制 PHYA 表达促进遮荫响应。
Shade-induced lncRNA PUAR promotes shade response by repressing PHYA expression.
机构信息
State Key Laboratory of Genetic Engineering, Institute of Plants Biology, School of Life Sciences, Fudan University, Shanghai, China.
出版信息
EMBO Rep. 2023 May 4;24(5):e56105. doi: 10.15252/embr.202256105. Epub 2023 Mar 27.
Shade avoidance syndrome (SAS) commonly occurs in plants experiencing vegetative shade, triggering a series of morphological and physiological changes for the plants to reach more light. A number of positive regulators, such as PHYTOCHROME-INTERACTING 7 (PIF7), and negative regulators, such as PHYTOCHROMES, are known to ensure appropriate SAS. Here, we identify 211 shade-regulated long non-coding RNAs (lncRNAs) in Arabidopsis. We further characterize PUAR (PHYA UTR Antisense RNA), a lncRNA produced from the intron of the 5' UTR of the PHYTOCHROME A (PHYA) locus. PUAR is induced by shade and promotes shade-induced hypocotyl elongation. PUAR physically associates with PIF7 and represses the shade-mediated induction of PHYA by blocking the binding of PIF7 to the 5' UTR of PHYA. Our findings highlight a role for lncRNAs in SAS and provide insight into the mechanism of PUAR in regulating PHYA gene expression and SAS.
避荫综合征（SAS）通常发生在经历营养遮荫的植物中，引发一系列植物形态和生理变化，以获得更多的光。已经知道许多正调控因子，如 PHYTOCHROME-INTERACTING 7（PIF7），和负调控因子，如 PHYTOCHROMES，以确保适当的 SAS。在这里，我们在拟南芥中鉴定了 211 个受遮荫调节的长非编码 RNA（lncRNA）。我们进一步表征了 PUAR（PHYA UTR Antisense RNA），一种从 PHYTOCHROME A（PHYA）基因座 5'UTR 内含子产生的 lncRNA。PUAR 受遮荫诱导，并通过阻止 PIF7 与 PHYA 的 5'UTR 结合来抑制 PIF7 介导的 PHYA 的诱导，从而促进遮荫诱导的下胚轴伸长。我们的研究结果强调了 lncRNA 在 SAS 中的作用，并为 lncRNA 在调节 PHYA 基因表达和 SAS 中的作用提供了新的见解。
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