Department of Public Health & Community Medicine, Tufts University School of Medicine, Tufts University, Boston, Massachusetts, USA.
Department of Basic & Translational Sciences, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
Int J Cancer. 2023 Jul 15;153(2):302-311. doi: 10.1002/ijc.34527. Epub 2023 Apr 9.
Periodontitis has been associated with an increased risk for gastrointestinal cancers. The objective of our study was to investigate the association of antibodies to oral bacteria and the risk of colon cancer in a cohort setting. Using the CLUE I cohort, a prospective cohort initiated in 1974 in Washington County, Maryland, we conducted a nested case-control study to examine the association of levels of IgG antibodies to 11 oral bacterial species (13 total strains) with risk of colon cancer diagnosed a median of 16 years later (range: 1-26 years). Antibody response was measured using checkerboard immunoblotting assays. We included 200 colon cancer cases and 200 controls matched on age, sex, cigarette smoking status, time of blood draw and pipe or cigar smoking status. Controls were selected using incidence density sampling. Conditional logistic regression models were used to assess the association between antibody levels and colon cancer risk. In the overall analysis, we observed significant inverse associations for 6 of the 13 antibodies measured (P-trends <.05) and one positive association for antibody levels to Aggregatibacter actinomycetemcomitans (ATCC 29523; P-trend = .04). While we cannot rule out a role for periodontal disease in colon cancer risk, findings from our study suggest that a strong adaptive immune response may be associated with a lower risk of colon cancer. More studies will need to examine whether the positive associations we observed with antibodies to A. actinomycetemcomitans reflect a true causal association for this bacterium.
牙周炎与胃肠道癌症的风险增加有关。我们的研究目的是在队列研究中调查口腔细菌抗体与结肠癌风险的关系。使用 CLUE I 队列，这是 1974 年在马里兰州华盛顿县启动的一项前瞻性队列研究，我们进行了一项嵌套病例对照研究，以检查针对 11 种口腔细菌（共 13 种菌株）的 IgG 抗体水平与中位数为 16 年后（范围：1-26 年）诊断出的结肠癌风险之间的关联。使用棋盘免疫印迹分析测定抗体反应。我们纳入了 200 例结肠癌病例和 200 例年龄、性别、吸烟状况、采血时间和烟斗或雪茄吸烟状况匹配的对照。对照采用发病率密度抽样选择。条件逻辑回归模型用于评估抗体水平与结肠癌风险之间的关联。在总体分析中，我们观察到 13 种抗体中有 6 种的抗体水平与结肠癌风险呈显著负相关（P 趋势<.05），而对伴放线放线杆菌（ATCC 29523；P 趋势=0.04）的抗体水平呈正相关。虽然我们不能排除牙周病在结肠癌风险中的作用，但我们的研究结果表明，强烈的适应性免疫反应可能与结肠癌风险降低有关。需要更多的研究来检验我们观察到的与伴放线放线杆菌抗体的阳性关联是否反映了该细菌的真正因果关系。
