DNA聚合酶的底物特异性。II. 5-(1-炔基)-dUTP作为克列诺夫DNA聚合酶的底物
Substrate specificity of DNA polymerases. II. 5-(1-Alkynyl)-dUTPs as substrates of the Klenow DNA polymerase enzyme.
作者信息
Otvös L, Szécsi J, Sági J, Kovács T
机构信息
Central Research Institute for Chemistry, Hungarian Academy of Sciences, Budapest.
出版信息
Nucleic Acids Symp Ser. 1987(18):125-9.
PMID:3697112
Abstract
A structure-activity relationship study was carried out with a sequence of 5-(1-alkynyl)-dUTPs in Klenow DNA polymerase enzyme-catalyzed reactions. Ability of modified dUTPs examined (up to the octynyl derivative) to serve as a substrate depended on both the length of substituent and the structure of DNA primer-template used.
摘要
在Klenow DNA聚合酶催化反应中，对一系列5-(1-炔基)-dUTP进行了构效关系研究。所检测的修饰dUTP（直至辛炔基衍生物）作为底物的能力取决于取代基的长度和所用DNA引物模板的结构。
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