[胆汁和胆汁酸对家兔、豚鼠和猪空肠中单胺氧化酶和乙酰胆碱酯酶活性的影响]

[Effect of bile and bile acids on monoamine oxidase and acetylcholinesterase activity in the jejunum of rabbits, guinea pigs and swine].

作者信息

Radymska-Wawrzyniak K, Studziński T

机构信息

Instytut Nauk Fizjologicznych AR w Lublinie.

出版信息

Pol Arch Weter. 1987;24(4):457-66.

PMID:3697337

Abstract

The studies were carried out in vitro on the jejunum of 10 pigs, 10 rabbits and 10 guinea pigs. The activity monoaminooxidase (MAO) was determined manometrically in Warburg's apparatus, that of acetylcholinesterase (AChE) spectrophotometrically after Ellman's method modified by urek and Syrowatka. The obtained results were statistically analysed. It was shown that in the animals studied the bile acids exerted a considerable effect on the activity of enzymes metabolising neurohormones. Under the influence of bile the activity of monoaminooxidase was inhibited. Stimulating, but less distinctly, bile effected the activity of acetylocholinesterase. Significant differences in the action of bile on the activity of these enzymes were found in the jejunum of the animal species studied. The highest decrease in the activity of MAO under the influence of bile was found in the jejunum of the rabbit (by about 65%), a weker in the pig (by 37.5%) and guinea-pig (by 18.5%). However, the highest increase in the activity of AChE under the influence of bile occurred in the pigs (by about 21.6%), a weaker in the rabbit (by 16.4%) and guinea-pig (by about 11.5%). Among the bile acids, the strongest action on both the activity of MAO and AChE was exerted by lithocholic acid, desoxycholic, weaker by cholic and chenodesoxycholic acid. MAO activity under the influence of lithocholic acid decreased (in the pig by about 63%, rabbit by 55.7% and guinea pig by 34.8%). After incubation of the jejunum in desoxycholic acid a decrease in MAO activity occurred (in the rabbit by 43.8%, pig by about 39.4% and guinea-pig by 15.7%). AChE activity under the influence of lithocholic acid increased (in the pig on average by 62.2% rabbit by 21.8 and guinea-pig by about 10.2%). After incubation of the jejunum in desoxycholic acid an increase in the activity of acetylcholinesteras occurred (in the pig by about 13.5%, guinea-pig by 7.7% rabbit 7.3%).

摘要

这些研究在10头猪、10只兔子和10只豚鼠的空肠上进行体外实验。单胺氧化酶（MAO）的活性通过瓦氏呼吸仪测压法测定，乙酰胆碱酯酶（AChE）的活性则按照Urek和Syrowatka改良后的埃尔曼方法用分光光度法测定。对所得结果进行统计学分析。结果表明，在所研究的动物中，胆汁酸对代谢神经激素的酶的活性有显著影响。在胆汁的影响下，单胺氧化酶的活性受到抑制。胆汁对乙酰胆碱酯酶的活性有刺激作用，但不太明显。在所研究的动物物种的空肠中，发现胆汁对这些酶活性的作用存在显著差异。在胆汁的影响下，兔子空肠中MAO活性下降幅度最大（约65%），猪空肠中下降幅度较小（37.5%），豚鼠空肠中下降幅度最小（18.5%）。然而，在胆汁的影响下，猪空肠中AChE活性增加幅度最大（约21.6%），兔子空肠中增加幅度较小（16.4%），豚鼠空肠中增加幅度最小（约11.5%）。在胆汁酸中，石胆酸对MAO和AChE的活性作用最强，脱氧胆酸作用次之，胆酸和鹅去氧胆酸作用较弱。在石胆酸的影响下，MAO活性下降（猪下降约63%，兔子下降55.7%，豚鼠下降34.8%）。空肠在脱氧胆酸中孵育后，MAO活性下降（兔子下降43.8%，猪下降约39.4%，豚鼠下降15.7%）。在石胆酸的影响下，AChE活性增加（猪平均增加62.2%，兔子增加21.8%，豚鼠增加约10.2%）。空肠在脱氧胆酸中孵育后，乙酰胆碱酯酶活性增加（猪增加约13.5%，豚鼠增加7.7%，兔子增加7.3%）。