预后生物标志物 SAAL1 对肺腺癌肿瘤生长的影响及其与免疫微环境的关系。

The effects of the prognostic biomarker SAAL1 on cancer growth and its association with the immune microenvironment in lung adenocarcinoma.

机构信息

Department of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.

Department of Obstetrics and Gynecology, Women and Children's Hospitalof, Chongqing Medical University, Chongqing 401147, China.

出版信息

BMC Cancer. 2023 Mar 27;23(1):275. doi: 10.1186/s12885-023-10741-5.

DOI:10.1186/s12885-023-10741-5

PMID:36973678

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10041717/

Abstract

BACKGROUND

Inhibition of Serum Amyloid A-like 1 (SAAL1) expression could inhibit cancer progression and improve the prognosis of cancer patients. At present, the correlation between SAAL1 and lung adenocarcinoma (LAC) remains unclear. Therefore, this study surveyed the worth and pathway of SAAL1 in LAC progression and immunity.

METHODS

Bioinformatics and immunohistochemistry were used to identify the SAAL1 expression in LAC. The roles of SAAL1 expression in the existence values of LAC patients were explored, and the nomograms were constructed. Clinical values of SAAL1 co-expressed genes were evaluated by COX regression, survival, and Receiver operating characteristic (ROC) analysis. EDU and western blotting methods were used to inquiry the functions and pathways of the SAAL1 in cell growths. The correlation between the SAAL1 level and immune microenvironment was visualized using correlation research.

RESULTS

SAAL1 level was elevated in LAC tissues, and was observed in cancer tissues of dead patients. SAAL1 overexpression had something to do with shorter overall survival, progression-free interval, and disease-specific survival in LAC. The area under the curve of SAAL1 was 0.902 in normal tissues and cancer tissues. Inhibition of SAAL1 expression could inhibit cancer cell proliferation, which may be related to the decreased expression of cyclin D1 and Bcl-2 proteins. In LAC, SAAL1 level had something to do with stromal, immune, and estimate scores, and correlated with macrophages, T cells, Th2 cells, CD8 T cells, NK CD56dim cells, DC, eosinophils, NK CD56bright cells, pDC, iDC, cytotoxic cells, Tgd, aDC cells, B cells, Tcm, and TFH levels. SAAL1 overexpression had something to do with existence values and the immunity in LAC.

CONCLUSIONS

Inhibition of SAAL1 expression could regulate cancer growth via cyclin D1 and Bcl-2. SAAL1 is a promising prognostic biomarker in LAC patients.

摘要

背景

抑制血清淀粉样蛋白 A 样蛋白 1（SAAL1）的表达可以抑制癌症的进展并改善癌症患者的预后。目前，SAAL1 与肺腺癌（LAC）之间的相关性尚不清楚。因此，本研究调查了 SAAL1 在 LAC 进展和免疫中的价值和途径。

方法

采用生物信息学和免疫组织化学方法鉴定 LAC 中的 SAAL1 表达。探讨 SAAL1 表达对 LAC 患者生存价值的作用，并构建列线图。通过 COX 回归、生存和受试者工作特征（ROC）分析评估 SAAL1 共表达基因的临床价值。采用 EDU 和 Western blot 方法探讨 SAAL1 在细胞生长中的作用及途径。采用相关性研究可视化 SAAL1 水平与免疫微环境的相关性。

结果

SAAL1 水平在 LAC 组织中升高，在死亡患者的癌组织中观察到。SAAL1 过表达与 LAC 患者的总生存期、无进展生存期和疾病特异性生存期缩短有关。SAAL1 在正常组织和癌组织中的曲线下面积分别为 0.902。抑制 SAAL1 表达可抑制癌细胞增殖，这可能与 cyclin D1 和 Bcl-2 蛋白表达降低有关。在 LAC 中，SAAL1 水平与基质、免疫和估计评分有关，与巨噬细胞、T 细胞、Th2 细胞、CD8 T 细胞、NK CD56dim 细胞、DC、嗜酸性粒细胞、NK CD56bright 细胞、pDC、iDC、细胞毒性细胞、Tgd、aDC 细胞、B 细胞、Tcm 和 TFH 水平有关。SAAL1 过表达与 LAC 患者的生存价值和免疫有关。