Kropacheva I V, Iudin Iu K, Shelud'ko N S
Biofizika. 1986 Mar-Apr;31(2):355-6.
An angle diagram of light scattering of myofibril suspensions was extremely asymmetric in the range of 0.05 to 10 degrees. The extent of asymmetry increased at relaxation and sharply decreased at contraction of myofibrils. Angle diagrams for myofibril light scattering calculated according to the approximate formulae of Mie theory, were close to those obtained in the experiment. It was shown that a high extent of asymmetry of calculated diagrams is determined by a low refractive index of myofibrils rather than by great dimensions of myofibrils. It is supposed that changes in the shape of scattering diagram and in other optical characteristics of myofibril and actomyosin suspensions associated with changes in their functional states depend mostly upon changes in the inner arrangement of particles rather than in their dimensions.
肌原纤维悬浮液的光散射角图在0.05至10度范围内极不对称。不对称程度在肌原纤维松弛时增加,在收缩时急剧下降。根据米氏理论近似公式计算的肌原纤维光散射角图与实验所得结果相近。结果表明,计算图的高度不对称性是由肌原纤维的低折射率而非其大尺寸决定的。据推测,与肌原纤维和肌动球蛋白悬浮液功能状态变化相关的散射图形状及其他光学特性的变化,主要取决于颗粒内部排列的变化而非其尺寸变化。