[CRISPR/Cas系统在病原体核酸检测中的应用：综述]
[Application of CRISPR/Cas systems in the nucleic acid detection of pathogens: a review].
作者信息
Li M, Qin Z, Yin K, Zheng B
机构信息
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Chinese Center for Tropical Diseases Research, National Health Commission Key Laboratory on Parasite and Vector Biology, WHO Collaborating Center for Tropical Diseases, National Center for International Research on Tropical Diseases, Ministry of Science and Technology, School of Global Health, Chinese Center for Tropical Diseases, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
Shanghai Jiao Tong University School of Medicine, China.
出版信息
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2023 Feb 13;35(1):98-103. doi: 10.16250/j.32.1374.2022227.
Rapid, sensitive and specific detection tools are critical for the prevention and control of infectious diseases. The nucleic acid amplification assays, including polymerase chain reaction and isothermal amplification technology, have been widely used for the detection of pathogens. Recently, nucleic acid detection-based on clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) have been developed, which are rapid, highly sensitive, highly specific, and portable. This review describes the classification and principle of CRISPR/Cas systems and their applications in pathogen detection, and discusses the prospects of CRISPR/Cas systems.
快速、灵敏且特异的检测工具对于传染病的预防和控制至关重要。核酸扩增检测方法，包括聚合酶链反应和等温扩增技术，已被广泛用于病原体检测。最近，基于成簇规律间隔短回文重复序列（CRISPR）/CRISPR相关蛋白（Cas）的核酸检测方法已被开发出来，这些方法快速、高度灵敏、高度特异且便于携带。本文综述了CRISPR/Cas系统的分类、原理及其在病原体检测中的应用，并探讨了CRISPR/Cas系统的前景。
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